Six-wave mixing coherent anti-Stokes Raman scattering microscopy

Autor(es)

Pelegati, Vitor B. ^{[1, 2]} ; Kyotoku, Bernardo B. C. ^[2] ; Padilha, Lazaro A. ^[2] ; Cesar, Carlos L. ^{[1, 2, 3]}

Afiliação do(s) autor(es)

^[1] Natl Inst Sci & Technol Photon Appl Cell Biol INF, Campinas, SP - Brazil

^[2] Univ Campinas UNICAMP, Gleb Wataghin Inst Phys, BR-13083859 Campinas, SP - Brazil

^[3] Univ Fed Ceara, Dept Phys, BR-60440900 Fortaleza, Ceara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BIOMEDICAL OPTICS EXPRESS; v. 9, n. 5, p. 2407-2417, MAY 1 2018.
• Citações Web of Science: 1

Resumo

Acquiring images of biological tissues and cells without the assistance of exogenous labels with a fast repetition rate and chemical specificity is what coherent anti-Stokes Raman Scattering (CARS) imaging offers. Nonresonant background (NRB) is one of the main drawbacks of the CARS microscopy technique because it limits the detection of weak Raman lines and the detection of low-concentration molecules. We show that a six-wave mixing process with two beams, which is a cascade effect of CARS, show better signal/NRB ratio and can be utilized for biological tissues imaging. The cascade CARS (CCARS) depends on chi-3 to the fourth power, instead of chi-3 squared as in the usual CARS signal; therefore, the contrast ratio with NRB is higher for CCARS than for CARS. We present analytic calculations showing that CCARS have better contrast over CARS in any situation. Comparison of the signals of both techniques generated on water-ethanol solutions confirm these results. Finally, we acquired CCARS images of fresh biological tissues, attesting that it is a useful tool for biological studies. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement (AU)

• Processo FAPESP: 05/51689-2 - Centro de Pesquisa em Óptica e Fotônica - UNICAMP (CEPOF-UNICAMP)
• Beneficiário: Hugo Luis Fragnito
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 13/16911-2 - Espectroscopia avançada em novos nanomateriais
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• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/50938-8 - INCT 2014: em Fotônica Aplicada à Biologia Celular INFABiC
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