Dietary advanced glycated end-products and medicines influence the expression of SIRT1 and DDOST in peripheral mononuclear cells from long-term type 1 diabetes patients

Autor(es):Mostrar menos -

Santos-Bezerra, Daniele P. ^[1] ; Machado-Lima, Adriana ^[2] ; Monteiro, Maria Beatriz ^[1] ; Admoni, Sharon N. ^[1] ; Perez, Ricardo V. ^[1] ; Machado, Cleide G. ^[3] ; Shimizu, Maria Heloiza ^[4] ; Cavaleiro, Ana M. ^[1] ; Thieme, Karina ^[1] ; Queiroz, Marcia S. ^[5] ; Machado, Ubiratan F. ^[6] ; Giannella-Neto, Daniel ^[7] ; Passarelli, Marisa ^[2] ; Correa-Giannella, Maria Lucia ^{[1, 7, 8]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FMUSP, Lab Carboidratos & Radioimunoensaios LIM 18, Fac Med, Sao Paulo - Brazil

^[2] Univ Sao Paulo FMUSP, Lab Lipides LIM 10, Fac Med, Sao Paulo - Brazil

^[3] Univ Sao Paulo HCFMUSP, Hosp Clin, Div Oftalmol, Fac Med, Sao Paulo - Brazil

^[4] Univ Sao Paulo FMUSP, Lab Pesquisa Basica Doencas Renais LIM 12, Fac Med, Sao Paulo - Brazil

^[5] Univ Sao Paulo HCFMUSP, Hosp Clin, Div Endocrinol, Fac Med, Sao Paulo - Brazil

^[6] Univ Sao Paulo, Inst Ciencias Biomed, Lab Metab & Endocrinol, Sao Paulo - Brazil

^[7] Univ Nove de Julho, Programa Posgrad Med, Sao Paulo - Brazil

^[8] Univ Sao Paulo, Nucleo Estudos & Terapia Celular & Mol NUCEL NETC, Fac Med, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Diabetes & Vascular Disease Research; v. 15, n. 1, p. 81-89, JAN 2018.
• Citações Web of Science: 3

Resumo

Quantitative polymerase chain reaction was employed to quantify expression of two genes coding for advanced glycation end-product receptors {[}RAGE (AGER) and AGER1 (DDOST)] and of the gene coding the deacetylase SIRT1 (SIRT1) in peripheral blood mononuclear cells from type 1 diabetes patients without {[}Group A, n = 35; 28.5 (24-39) years old; median (interquartile interval)] or with at least one microvascular complication {[}Group B, n = 117; 34.5 (30-42) years old]; 31 healthy controls were also included. In a subgroup of 48 patients, daily advanced glycation end-products intake before blood collection was assessed. Lower expression of DDOST was found in patients than in controls after adjustment for sex, age, use of statins, angiotensin-converting enzyme inhibitors and angiotensin receptor blockers. Higher expressions of AGER, DDOST and SIRT1 were observed in Group A. Stratifying by complications, AGER and DDOST expressions were higher in those without retinopathy and without diabetic kidney disease, respectively, compared to patients with these complications. Patients using statins or angiotensin receptor blockers presented higher expression of DDOST. Expression of SIRT1 was higher in patients consuming >= 12,872 KU daily of advanced glycation end-products. Although AGER, DDOST and SIRT1 are differently expressed in peripheral blood mononuclear cells from type 1 diabetes patients with and without microvascular complications, they are also influenced by dietary advanced glycation end-products and by statins and angiotensin receptor blockers. (AU)

• Processo FAPESP: 12/04831-1 - Novos moduladores do controle glicêmico e do desenvolvimento de complicações crônicas no Diabetes mellitus: perspectivas preventivas e terapêuticas
• Beneficiário: Ubiratan Fabres Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/15603-0 - Desvendando mecanismos envolvidos no controle glicêmico e nas complicações crônicas do Diabetes mellitus: contribuições à saúde humana
• Beneficiário: Ubiratan Fabres Machado
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/25490-8 - A menor expressão do RNA mensageiro do receptor 1 de produtos finais de glicação avançada (AGER1) em células linfomononucleares de sangue periférico está associada à doença renal em portadores de diabetes mellitus tipo 1
• Beneficiário: Daniele Pereira dos Santos Bezerra
• Modalidade de apoio: Bolsas no Brasil - Mestrado