Genome-wide analysis of the human malaria parasite Plasmodium falciparum transcription factor PfNF-YB shows interaction with a CCAAT motif

Autor(es)

Lima, Wania Rezende ^{[1, 2]} ; Martins, David Correa ^[3] ; Parreira, Kleber Simonio ^{[4, 2]} ; Scarpelli, Pedro ^[1] ; de Moraes, Miriam Santos ^[1] ; Topalis, Pantelis ^[5] ; Hashimoto, Ronaldo Fumio ^[6] ; Garcia, Celia R. S. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil

^[2] Univ Fed Mato Grosso, Inst Ciencias Exatas & Nat Med, Campus Rondonopolis, Cuiaba, Mato Grosso - Brazil

^[3] Univ Fed ABC, Ctr Matemat Comp & Cognicao, Santo Andre - Brazil

^[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[5] FORTH, Inst Mol Biol & Biotechnol, Hellas - Greece

^[6] Univ Sao Paulo, Inst Matemat & Estat, Dept Ciencia Comp, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ONCOTARGET; v. 8, n. 69, p. 113987-114001, DEC 26 2017.
• Citações Web of Science: 1

Resumo

Little is known about transcription factor regulation during the Plasmodium falciparum intraerythrocytic cycle. In order to elucidate the role of the P. falciparum (Pf)NF-YB transcription factor we searched for target genes in the entire genome. PfNF-YB mRNA is highly expressed in late trophozoite and schizont stages relative to the ring stage. In order to determine the candidate genes bound by PfNF-YB a ChIP-on-chip assay was carried out and 297 genes were identified. Ninety nine percent of PfNF-YB binding was to putative promoter regions of protein coding genes of which only 16% comprise proteins of known function. Interestingly, our data reveal that PfNF-YB binding is not exclusively to a canonical CCAAT box motif. PfNF-YB binds to genes coding for proteins implicated in a range of different biological functions, such as replication protein A large subunit (DNA replication), hypoxanthine phosphoribosyltransferase (nucleic acid metabolism) and multidrug resistance protein 2 (intracellular transport). (AU)

• Processo FAPESP: 15/01587-0 - Armazenagem, modelagem e análise de sistemas dinâmicos para aplicações em e-Science
• Beneficiário: João Eduardo Ferreira
• Modalidade de apoio: Auxílio à Pesquisa - Programa eScience e Data Science - Temático
• Processo FAPESP: 11/51295-5 - Genômica funcional em Plasmodium
• Beneficiário: Célia Regina da Silva Garcia
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/50761-2 - Modelos e métodos de e-Science para ciências da vida e agrárias
• Beneficiário: Roberto Marcondes Cesar Junior
• Modalidade de apoio: Auxílio à Pesquisa - Temático