Genome-wide analysis of the human malaria parasite Plasmodium falciparum transcription factor PfNF-YB shows interaction with a CCAAT motif

Author(s)

Lima, Wania Rezende ^{[1, 2]} ; Martins, David Correa ^[3] ; Parreira, Kleber Simonio ^{[4, 2]} ; Scarpelli, Pedro ^[1] ; de Moraes, Miriam Santos ^[1] ; Topalis, Pantelis ^[5] ; Hashimoto, Ronaldo Fumio ^[6] ; Garcia, Celia R. S. ^[1]

Affiliation

^[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil

^[2] Univ Fed Mato Grosso, Inst Ciencias Exatas & Nat Med, Campus Rondonopolis, Cuiaba, Mato Grosso - Brazil

^[3] Univ Fed ABC, Ctr Matemat Comp & Cognicao, Santo Andre - Brazil

^[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil

^[5] FORTH, Inst Mol Biol & Biotechnol, Hellas - Greece

^[6] Univ Sao Paulo, Inst Matemat & Estat, Dept Ciencia Comp, Sao Paulo - Brazil

• Document type: Journal article
• Source: ONCOTARGET; v. 8, n. 69, p. 113987-114001, DEC 26 2017.
• Web of Science Citations: 1

Abstract

Little is known about transcription factor regulation during the Plasmodium falciparum intraerythrocytic cycle. In order to elucidate the role of the P. falciparum (Pf)NF-YB transcription factor we searched for target genes in the entire genome. PfNF-YB mRNA is highly expressed in late trophozoite and schizont stages relative to the ring stage. In order to determine the candidate genes bound by PfNF-YB a ChIP-on-chip assay was carried out and 297 genes were identified. Ninety nine percent of PfNF-YB binding was to putative promoter regions of protein coding genes of which only 16% comprise proteins of known function. Interestingly, our data reveal that PfNF-YB binding is not exclusively to a canonical CCAAT box motif. PfNF-YB binds to genes coding for proteins implicated in a range of different biological functions, such as replication protein A large subunit (DNA replication), hypoxanthine phosphoribosyltransferase (nucleic acid metabolism) and multidrug resistance protein 2 (intracellular transport). (AU)

• FAPESP's process: 15/01587-0 - Storage, modeling and analysis of dynamical systems for e-Science applications
• Grantee: João Eduardo Ferreira
• Support Opportunities: Research Grants - eScience and Data Science Program - Thematic Grants
• FAPESP's process: 11/51295-5 - Functional genomics in Plasmodium
• Grantee: Célia Regina da Silva Garcia
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 11/50761-2 - Models and methods of e-Science for life and agricultural sciences
• Grantee: Roberto Marcondes Cesar Junior
• Support Opportunities: Research Projects - Thematic Grants