| Full text | |
| Author(s): |
Lima, Wania Rezende
[1, 2]
;
Martins, David Correa
[3]
;
Parreira, Kleber Simonio
[4, 2]
;
Scarpelli, Pedro
[1]
;
de Moraes, Miriam Santos
[1]
;
Topalis, Pantelis
[5]
;
Hashimoto, Ronaldo Fumio
[6]
;
Garcia, Celia R. S.
[1]
Total Authors: 8
|
| Affiliation: | [1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil
[2] Univ Fed Mato Grosso, Inst Ciencias Exatas & Nat Med, Campus Rondonopolis, Cuiaba, Mato Grosso - Brazil
[3] Univ Fed ABC, Ctr Matemat Comp & Cognicao, Santo Andre - Brazil
[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
[5] FORTH, Inst Mol Biol & Biotechnol, Hellas - Greece
[6] Univ Sao Paulo, Inst Matemat & Estat, Dept Ciencia Comp, Sao Paulo - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | ONCOTARGET; v. 8, n. 69, p. 113987-114001, DEC 26 2017. |
| Web of Science Citations: | 1 |
| Abstract | |
Little is known about transcription factor regulation during the Plasmodium falciparum intraerythrocytic cycle. In order to elucidate the role of the P. falciparum (Pf)NF-YB transcription factor we searched for target genes in the entire genome. PfNF-YB mRNA is highly expressed in late trophozoite and schizont stages relative to the ring stage. In order to determine the candidate genes bound by PfNF-YB a ChIP-on-chip assay was carried out and 297 genes were identified. Ninety nine percent of PfNF-YB binding was to putative promoter regions of protein coding genes of which only 16% comprise proteins of known function. Interestingly, our data reveal that PfNF-YB binding is not exclusively to a canonical CCAAT box motif. PfNF-YB binds to genes coding for proteins implicated in a range of different biological functions, such as replication protein A large subunit (DNA replication), hypoxanthine phosphoribosyltransferase (nucleic acid metabolism) and multidrug resistance protein 2 (intracellular transport). (AU) | |
| FAPESP's process: | 15/01587-0 - Storage, modeling and analysis of dynamical systems for e-Science applications |
| Grantee: | João Eduardo Ferreira |
| Support Opportunities: | Research Grants - eScience and Data Science Program - Thematic Grants |
| FAPESP's process: | 11/51295-5 - Functional genomics in Plasmodium |
| Grantee: | Célia Regina da Silva Garcia |
| Support Opportunities: | Research Projects - Thematic Grants |
| FAPESP's process: | 11/50761-2 - Models and methods of e-Science for life and agricultural sciences |
| Grantee: | Roberto Marcondes Cesar Junior |
| Support Opportunities: | Research Projects - Thematic Grants |