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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Genome-wide analysis of the human malaria parasite Plasmodium falciparum transcription factor PfNF-YB shows interaction with a CCAAT motif

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Author(s):
Lima, Wania Rezende [1, 2] ; Martins, David Correa [3] ; Parreira, Kleber Simonio [4, 2] ; Scarpelli, Pedro [1] ; de Moraes, Miriam Santos [1] ; Topalis, Pantelis [5] ; Hashimoto, Ronaldo Fumio [6] ; Garcia, Celia R. S. [1]
Total Authors: 8
Affiliation:
[1] Univ Sao Paulo, Inst Biociencias, Dept Fisiol, Sao Paulo - Brazil
[2] Univ Fed Mato Grosso, Inst Ciencias Exatas & Nat Med, Campus Rondonopolis, Cuiaba, Mato Grosso - Brazil
[3] Univ Fed ABC, Ctr Matemat Comp & Cognicao, Santo Andre - Brazil
[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
[5] FORTH, Inst Mol Biol & Biotechnol, Hellas - Greece
[6] Univ Sao Paulo, Inst Matemat & Estat, Dept Ciencia Comp, Sao Paulo - Brazil
Total Affiliations: 6
Document type: Journal article
Source: ONCOTARGET; v. 8, n. 69, p. 113987-114001, DEC 26 2017.
Web of Science Citations: 1
Abstract

Little is known about transcription factor regulation during the Plasmodium falciparum intraerythrocytic cycle. In order to elucidate the role of the P. falciparum (Pf)NF-YB transcription factor we searched for target genes in the entire genome. PfNF-YB mRNA is highly expressed in late trophozoite and schizont stages relative to the ring stage. In order to determine the candidate genes bound by PfNF-YB a ChIP-on-chip assay was carried out and 297 genes were identified. Ninety nine percent of PfNF-YB binding was to putative promoter regions of protein coding genes of which only 16% comprise proteins of known function. Interestingly, our data reveal that PfNF-YB binding is not exclusively to a canonical CCAAT box motif. PfNF-YB binds to genes coding for proteins implicated in a range of different biological functions, such as replication protein A large subunit (DNA replication), hypoxanthine phosphoribosyltransferase (nucleic acid metabolism) and multidrug resistance protein 2 (intracellular transport). (AU)

FAPESP's process: 11/51295-5 - Functional genomics in Plasmodium
Grantee:Célia Regina da Silva Garcia
Support type: Research Projects - Thematic Grants
FAPESP's process: 11/50761-2 - Models and methods of e-Science for life and agricultural sciences
Grantee:Roberto Marcondes Cesar Junior
Support type: Research Projects - Thematic Grants
FAPESP's process: 15/01587-0 - Storage, modeling and analysis of dynamical systems for e-Science applications
Grantee:João Eduardo Ferreira
Support type: Research Grants - eScience and Data Science Program - Thematic Grants