New findings from the first transcriptome of the Bothrops moojeni snake venom gland

Autor(es)

Amorim, Fernanda Gobbi ^[1] ; Morandi-Filho, Romualdo ^[2] ; Fujimura, Patricia Tieme ^[2] ; Ueira-Vieira, Carlos ^[2] ; Sampaio, Suely Vilela ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Lab Toxinol, Ribeirao Preto, SP - Brazil

^[2] Univ Fed Uberlandia, Inst Genet & Biochem, Lab Genet, Uberlandia, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Toxicon; v. 140, p. 105-117, DEC 15 2017.
• Citações Web of Science: 8

Resumo

Snakebites are a serious health problem in tropical countries. In Brazil, the genus Bothrops (Viperidae family) causes most of the ophidic accidents, characterized by proteolysis and haemorrhage. Snake venoms are rich sources of toxins with great therapeutic and biotechnological potential and omits approaches is a valuable tool for identification of new bioactive components in the venom. In this study, we described the first transcriptome of the venom gland of Bothrops moojeni snake, using the next generation sequencing with the Illumina platform. We identified: (i) 20 venom components classes, among which metalloproteases were the most expressed ones, followed by serine proteases and phospholipases; and (ii) the 33 full-length amino acid sequences of toxins that have never been reported before in B. moojeni venom, such as one cysteine-rich secretory protein (Moojin), two hyaluronidases (BmooHyal-1 and BmooHyal-2), and one three-finger toxin (Bmoo-3FTx). Altogether, the transcripts identified herein represent a starting point for the analysis of structure-function relationships of toxins, which shall help develop novel biological tools and therapeutic drugs. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 16/20641-9 - Integração da abordagens ômicas para obter o perfil da peçonha da serpente Bothrops moojeni: revelando novas toxinas e divergências ontogênicas
• Beneficiário: Fernanda Gobbi Amorim
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado
• Processo FAPESP: 15/26609-7 - Venômica da serpente Bothrops moojeni: aplicação de toxinas de interesse biotecnológico obtidas por técnicas ômicas
• Beneficiário: Fernanda Gobbi Amorim
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/23236-4 - Toxinas animais nativas e recombinantes: análise funcional, estrutural e molecular
• Beneficiário: Suely Vilela
• Modalidade de apoio: Auxílio à Pesquisa - Temático