New findings from the first transcriptome of the Bothrops moojeni snake venom gland

Author(s)

Amorim, Fernanda Gobbi ^[1] ; Morandi-Filho, Romualdo ^[2] ; Fujimura, Patricia Tieme ^[2] ; Ueira-Vieira, Carlos ^[2] ; Sampaio, Suely Vilela ^[1]

Affiliation

^[1] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Dept Clin Anal Toxicol & Food Sci, Lab Toxinol, Ribeirao Preto, SP - Brazil

^[2] Univ Fed Uberlandia, Inst Genet & Biochem, Lab Genet, Uberlandia, MG - Brazil

• Document type: Journal article
• Source: Toxicon; v. 140, p. 105-117, DEC 15 2017.
• Web of Science Citations: 8

Abstract

Snakebites are a serious health problem in tropical countries. In Brazil, the genus Bothrops (Viperidae family) causes most of the ophidic accidents, characterized by proteolysis and haemorrhage. Snake venoms are rich sources of toxins with great therapeutic and biotechnological potential and omits approaches is a valuable tool for identification of new bioactive components in the venom. In this study, we described the first transcriptome of the venom gland of Bothrops moojeni snake, using the next generation sequencing with the Illumina platform. We identified: (i) 20 venom components classes, among which metalloproteases were the most expressed ones, followed by serine proteases and phospholipases; and (ii) the 33 full-length amino acid sequences of toxins that have never been reported before in B. moojeni venom, such as one cysteine-rich secretory protein (Moojin), two hyaluronidases (BmooHyal-1 and BmooHyal-2), and one three-finger toxin (Bmoo-3FTx). Altogether, the transcripts identified herein represent a starting point for the analysis of structure-function relationships of toxins, which shall help develop novel biological tools and therapeutic drugs. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 16/20641-9 - Integration of Omics Approaches to Profile the Venom of the Bothrops moojeni snake: unreavelling new toxins and ontogenic divergences
• Grantee: Fernanda Gobbi Amorim
• Support Opportunities: Scholarships abroad - Research Internship - Post-doctor
• FAPESP's process: 15/26609-7 - Bothrops moojeni snake venomics: application of toxins of biotechnological interest obtained by omics techniques
• Grantee: Fernanda Gobbi Amorim
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• FAPESP's process: 11/23236-4 - Native and recombinant animal toxins: functional, structural and molecular analysis
• Grantee: Suely Vilela
• Support Opportunities: Research Projects - Thematic Grants