Polyphenol-rich green tea extract improves adipose tissue metabolism by down-regulating miR-335 expression and mitigating insulin resistance and inflammation

Autor(es)

Otton, Rosemari ^[1] ; Bolin, Anaysa Paola ^[2] ; Ferreira, Leticia Torres ^[1] ; Marinovic, Marcelo Paradiso ^[1] ; Silva Rocha, Andrea Livia ^[3] ; Mori, Marcelo Alves ^[3]

Afiliação do(s) autor(es)

^[1] Univ Cruzeiro Sul, CBS, Interdisciplinary Postgrad Program Hlth Sci, BR-03342000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Biomed Sci Inst, Dept Pharmacol, BR-05508000 Sao Paulo - Brazil

^[3] Univ Estadual Campinas, Dept Biochem & Tissue Biol, BR-13083862 Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF NUTRITIONAL BIOCHEMISTRY; v. 57, p. 170-179, JUL 2018.
• Citações Web of Science: 11

Resumo

Obesity leads to changes in miRNA expression in adipose tissue, and this modulation is linked to the pathophysiology of the disease. Green tea (GT) is a natural source of polyphenols that have been shown to confer health benefits, particularly preventing metabolic diseases. Here, we investigated if the beneficial effects of GT in obesity results from changes in the miRNA profile in white adipose tissue. GT treatment {[}500 mg/body weight (BW)/12 weeks] increased energy expenditure of high-fat diet-fed mice (16 weeks), leading to reduced weight gain, decreased adiposity, reduced inflammation and improved insulin sensitivity. These phenotypes were associated with a decrease in the expression of miR-335 in the adipose tissue. miR-335 was up-regulated by TNF-alpha in adipocytes and, in turn, down-regulated genes involved in insulin signaling and lipid metabolism. On the other hand, GT inhibited TNF-a effect. In conclusion, miR-335 serves as a link between inflammation and impaired metabolism in adipose tissue, providing an important mechanistic insight into the molecular basis underlying GT action during obesity. (C) 2018 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 13/22293-0 - Identificação de miRNAs e validação de genes-alvo no tecido adiposo e fígado de camundongos obesos em resposta ao tratamento com extrato de chá verde
• Beneficiário: Rosemari Otton
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/01316-7 - Dicer, miRNAs e o controle da função mitocondrial no contexto do envelhecimento e da restrição calórica
• Beneficiário: Marcelo Alves da Silva Mori
• Linha de fomento: Auxílio à Pesquisa - Regular