B-1 cells and B-1 cell precursors prompt different responses to Wnt signaling

Autor(es)

Osugui, Lika ^[1] ; de Roo, Jolanda J. ^[2] ; de Oliveira, Vivian Cristina ^[1] ; Pires Sodre, Ana Clara ^[1] ; Staal, Frank J. T. ^[2] ; Popi, Ana Flavia ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, Sao Paulo - Brazil

^[2] Leiden Univ, Dept Immunohematol & Blood Transfus, Med Ctr, Leiden - Netherlands

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 13, n. 6 JUN 21 2018.
• Citações Web of Science: 2

Resumo

Recently several studies demonstrated a role for the Wnt pathway in lymphocyte development and self-renewal of hematopoietic stem cells (HSCs). B-1 cells constitute a separate lineage of B lymphocytes, originating during fetal hematopoiesis, expressing lymphoid and myeloid markers and possessing self-renewal ability, similar to early hematopoietic progenitors and HSCs. A plethora of studies have shown an important role for the evolutionary conserved Wnt pathway in the biology of HSCs and T lymphocyte development. Our previous data demonstrated abundant expression of Wnt pathway components by B-1 cells, including Wnt ligands and receptors. Here we report that the canonical Wnt pathway is activated in B-1 cell precursors, but not in mature B-1 cells. However, both B-1 precursors and B-1 cells are able to respond to Wnt ligands in vitro. Canonical Wnt activity promotes proliferation of B-1 cells, while non-canonical Wnt signals induce the expansion of B-1 precursors. Interestingly, using a co-culture system with OP9 cells, Wnt3a stimulus supported the generation of B-1 a cells. Taking together, these results indicate that B-1 cells and their progenitors are differentially responsive to Wnt ligands, and that the balance of activation of canonical and non-canonical Wnt signaling may regulate the maintenance and differentiation of different B-1 cell subsets. (AU)

• Processo FAPESP: 08/55526-9 - Participação da via Wnt/beta-catenina na diferenciação e auto-renovação de células B-1
• Beneficiário: Ana Flavia Popi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 11/23464-7 - Análise do potencial de diferenciação linfóide e mielóide de células B-1 in vitro: papel da via de sinalização Wnt/beta-catenina
• Beneficiário: Lika Osugui
• Modalidade de apoio: Bolsas no Brasil - Doutorado