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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

B-1 cells and B-1 cell precursors prompt different responses to Wnt signaling

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Author(s):
Osugui, Lika [1] ; de Roo, Jolanda J. [2] ; de Oliveira, Vivian Cristina [1] ; Pires Sodre, Ana Clara [1] ; Staal, Frank J. T. [2] ; Popi, Ana Flavia [1]
Total Authors: 6
Affiliation:
[1] Univ Fed Sao Paulo, Dept Microbiol Imunol & Parasitol, Escola Paulista Med, Sao Paulo - Brazil
[2] Leiden Univ, Dept Immunohematol & Blood Transfus, Med Ctr, Leiden - Netherlands
Total Affiliations: 2
Document type: Journal article
Source: PLoS One; v. 13, n. 6 JUN 21 2018.
Web of Science Citations: 2
Abstract

Recently several studies demonstrated a role for the Wnt pathway in lymphocyte development and self-renewal of hematopoietic stem cells (HSCs). B-1 cells constitute a separate lineage of B lymphocytes, originating during fetal hematopoiesis, expressing lymphoid and myeloid markers and possessing self-renewal ability, similar to early hematopoietic progenitors and HSCs. A plethora of studies have shown an important role for the evolutionary conserved Wnt pathway in the biology of HSCs and T lymphocyte development. Our previous data demonstrated abundant expression of Wnt pathway components by B-1 cells, including Wnt ligands and receptors. Here we report that the canonical Wnt pathway is activated in B-1 cell precursors, but not in mature B-1 cells. However, both B-1 precursors and B-1 cells are able to respond to Wnt ligands in vitro. Canonical Wnt activity promotes proliferation of B-1 cells, while non-canonical Wnt signals induce the expansion of B-1 precursors. Interestingly, using a co-culture system with OP9 cells, Wnt3a stimulus supported the generation of B-1 a cells. Taking together, these results indicate that B-1 cells and their progenitors are differentially responsive to Wnt ligands, and that the balance of activation of canonical and non-canonical Wnt signaling may regulate the maintenance and differentiation of different B-1 cell subsets. (AU)

FAPESP's process: 11/23464-7 - Analysis of lymphoid and myeloid differentiation potential of B-1 cells in vitro: role of Wnt/beta-catenin signaling pathway
Grantee:Lika Osugui
Support Opportunities: Scholarships in Brazil - Doctorate