Hematopoietic Stem Cells are pluripotent cells, which are self-renewing and able to give rise to all lineages of the blood. Although intracellular factors and extrinsic signals play an important role in differentiation of HSC to mature blood cells, the complete mechanism of cellular commitment remains unclear. Wnt signaling pathway is known to regulate many fundamental developmental processes, it is also involved in embryonic cells development and commitment, in addition to be highly conserved during evolution. Recently, studies demonstrated the presence of signal transduction by Wnt pathway in lymphocyte development and HSC self-renewal. Data from our group have showed that B-1 cells express components of Wnt/beta-catenin pathway, like Wnt ligants and receptors. We suggested that Wnt/beta-catenin pathway plays an important role in B-1 self-renewal in vitro, and its inhibition could induce myeloid differentiation in B-1 cells. In order to understand the implications of Wnt pathway in B-1 cells differentiation in vitro, this work aims to investigate the effects of canonical and non-canonical activation of Wnt/beta-catenin pathway in this process, using Wnt ligants, Wnt3a and Wnt5a.
News published in Agência FAPESP Newsletter about the scholarship: