| Texto completo | |
| Autor(es): |
Leaden, Laura
[1]
;
Silva, Larissa G.
[1]
;
Ribeiro, Rodolfo A.
[1]
;
dos Santos, Naara M.
[1]
;
Lorenzetti, Alan P. R.
[2]
;
Alegria, Thiago G. P.
[3]
;
Schulz, Mariane L.
[4]
;
Medeiros, Marisa H. G.
[4]
;
Koide, Tie
[2]
;
Marques, V, Marilis
Número total de Autores: 10
|
| Afiliação do(s) autor(es): | [1] V, Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo - Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Bioquim & Imunol, Ribeirao Preto - Brazil
[3] Univ Sao Paulo, Inst Biociencias, Dept Genet & Biol Evolut, Sao Paulo - Brazil
[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo - Brazil
Número total de Afiliações: 4
|
| Tipo de documento: | Artigo Científico |
| Fonte: | FRONTIERS IN MICROBIOLOGY; v. 9, AUG 28 2018. |
| Citações Web of Science: | 5 |
| Resumo | |
In C. crescentus, iron metabolism is mainly controlled by the transcription factor Fur (ferric uptake regulator). Iron-bound Fur represses genes related to iron uptake and can directly activate the expression of genes for iron-containing proteins. In this work, we used total RNA sequencing (RNA-seq) of wild type C. crescentus growing in minimal medium under iron limitation and a fur mutant strain to expand the known Fur regulon, and to identify novel iron-regulated genes. The RNA-seq of cultures treated with the iron chelator 2-2-dypiridyl (DP) allowed identifying 256 upregulated genes and 236 downregulated genes, being 176 and 204 newly identified, respectively. Sixteen transcription factors and seven sRNAs were upregulated in iron limitation, suggesting that the response to low iron triggers a complex regulatory network. Notably, lexA along with most of its target genes were upregulated, suggesting that DP treatment caused DNA damage, and the SOS DNA repair response was activated in a RecA-dependent manner, as confirmed by RT-qPCR. Fluorescence microscopy assays using an oxidation-sensitive dye showed that wild type cells in iron limitation and the fur mutant were under endogenous oxidative stress, and a direct measurement of cellular H2O2 showed that cells in iron-limited media present a higher amount of endogenous H2O2. A mutagenesis assay using the rpoB gene as a reporter showed that iron limitation led to an increase in the mutagenesis rate. These results showed that iron deficiency causes C. crescentus cells to suffer oxidative stress and to activate the SOS response, indicating an increase in DNA damage. (AU) | |
| Processo FAPESP: | 14/04046-8 - Sistemas regulatórios da resposta bacteriana a estresses |
| Beneficiário: | Marilis Do Valle Marques |
| Modalidade de apoio: | Auxílio à Pesquisa - Temático |
| Processo FAPESP: | 16/06378-3 - Caracterização da DEAD box-RNA helicase CC1478 em Caulobacter crescentus e sua regulação |
| Beneficiário: | Rodolfo Alvarenga Ribeiro |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 15/07386-7 - Identificação de RNAs regulatórios em resposta à disponibilidade de ferro e seus mecanismos de ação em Caulobacter crescentus |
| Beneficiário: | Laura Leaden |
| Modalidade de apoio: | Bolsas no Brasil - Pós-Doutorado |
| Processo FAPESP: | 17/02127-9 - Caracterização de sistemas de transporte de ferro em Caulobacter crescentus |
| Beneficiário: | Naara Marcondes dos Santos |
| Modalidade de apoio: | Bolsas no Brasil - Mestrado |
| Processo FAPESP: | 15/21038-1 - O transcritoma antisense da archaea halofílica Halobacterium salinarum |
| Beneficiário: | Tie Koide |
| Modalidade de apoio: | Auxílio à Pesquisa - Regular |
| Processo FAPESP: | 17/03052-2 - RNAs não codificantes derivados de sequências de inserção na arqueia halófila Halobacterium salinarum NRC-1 |
| Beneficiário: | Alan Péricles Rodrigues Lorenzetti |
| Modalidade de apoio: | Bolsas no Brasil - Doutorado |