Intake of Protein Hydrolysates and Phenolic Fractions Isolated from Flaxseed Ameliorates TNBS-Induced Colitis

Autor(es)

Silva, Fernanda G. D. E. ^{[1, 2]} ; Paiatto, Lisiery N. ^{[3, 4]} ; Yamada, Aureo T. ^[5] ; Netto, Flavia M. ^[1] ; Simioni, Patricia U. ^{[3, 4, 6]} ; Tamashiro, Wirla M. S. C. ^[3]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, UNICAMP, Sch Food Engn, Dept Food & Nutr, BR-13083862 Campinas, SP - Brazil

^[2] Univ Fed Ouro Preto, Sch Nutr, Dept Food, BR-3540000 Ouro Preto - Brazil

^[3] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Genet Evolut Microbiol & Immunol, BR-13083970 Campinas, SP - Brazil

^[4] Univ Estadual Paulista, UNESP, Inst Biosci, Dept Biochem & Immunol, BR-13506900 Rio Claro - Brazil

^[5] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13506970 Campinas, SP - Brazil

^[6] Fac Amer FAM, Dept Biomed Sci, BR-13477360 Americana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MOLECULAR NUTRITION & FOOD RESEARCH; v. 62, n. 17 SEP 2018.
• Citações Web of Science: 3

Resumo

Scope: In the attempt to develop new therapeutic treatments for colitis, fractions containing phenolic compound isolate (Phi) and phenolic reduced-flaxseed protein hydrolysate (phr-FPH) from flaxseed are evaluated for their effects on the in vitro production of pro-inflammatory mediators and on the course of experimental colitis. Methods and results: The anti-inflammatory effects of Phi and phr-FPH from flaxseeds are studied in RAW264.7 cells and in trinitrobenzene sulphonic acid (TNBS) colitis model. It is observed that the incubation with Phi or phr-FPH result in lower levels of tumor necrosis factor and nitric oxide in macrophages stimulated with bacterial lipopolysaccharide + interferon-gamma. Prophylactic and therapeutic treatments with Phi and phr-FPH, respectively, greatly contribute to the prevention of weight loss and colon inflammation in colitic BALB/c mice. T cell proliferation, expansion of TH1 and TH17 cells, and pro-inflammatory cytokines are lower, whereas Treg cells are higher in spleen cell cultures from Phi-treated mice. In addition, therapeutic phr-FPH treatment is able to reduce the expansion of TH17 in splenic cell cultures. Conclusion: The consumption of phenolic and protein compounds extracted from flaxseeds has a protective effect on TNBS-induced colitis, and may be useful in the control of other inflammatory disorders. (AU)

• Processo FAPESP: 10/52680-7 - Atividade antioxidante e bioacessibilidade na forma livre e em emulsao de hidrolisados de linhaca (linum usitatissimum l.);
• Beneficiário: Flavia Maria Netto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/08591-0 - Avaliação dos efeitos da tolerância oral sobre o curso da resposta imune na colite experimental
• Beneficiário: Lisiery Negrini Paiatto
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 13/20258-2 - Efeitos da tolerância oral e da administração de células dendríticas sobre a resposta imune na colite experimental
• Beneficiário: Patricia Ucelli Simioni
• Modalidade de apoio: Auxílio à Pesquisa - Regular