| Full text | |
| Author(s): |
Silva, Fernanda G. D. E.
[1, 2]
;
Paiatto, Lisiery N.
[3, 4]
;
Yamada, Aureo T.
[5]
;
Netto, Flavia M.
[1]
;
Simioni, Patricia U.
[3, 4, 6]
;
Tamashiro, Wirla M. S. C.
[3]
Total Authors: 6
|
| Affiliation: | [1] Univ Estadual Campinas, UNICAMP, Sch Food Engn, Dept Food & Nutr, BR-13083862 Campinas, SP - Brazil
[2] Univ Fed Ouro Preto, Sch Nutr, Dept Food, BR-3540000 Ouro Preto - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Genet Evolut Microbiol & Immunol, BR-13083970 Campinas, SP - Brazil
[4] Univ Estadual Paulista, UNESP, Inst Biosci, Dept Biochem & Immunol, BR-13506900 Rio Claro - Brazil
[5] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13506970 Campinas, SP - Brazil
[6] Fac Amer FAM, Dept Biomed Sci, BR-13477360 Americana - Brazil
Total Affiliations: 6
|
| Document type: | Journal article |
| Source: | MOLECULAR NUTRITION & FOOD RESEARCH; v. 62, n. 17 SEP 2018. |
| Web of Science Citations: | 3 |
| Abstract | |
Scope: In the attempt to develop new therapeutic treatments for colitis, fractions containing phenolic compound isolate (Phi) and phenolic reduced-flaxseed protein hydrolysate (phr-FPH) from flaxseed are evaluated for their effects on the in vitro production of pro-inflammatory mediators and on the course of experimental colitis. Methods and results: The anti-inflammatory effects of Phi and phr-FPH from flaxseeds are studied in RAW264.7 cells and in trinitrobenzene sulphonic acid (TNBS) colitis model. It is observed that the incubation with Phi or phr-FPH result in lower levels of tumor necrosis factor and nitric oxide in macrophages stimulated with bacterial lipopolysaccharide + interferon-gamma. Prophylactic and therapeutic treatments with Phi and phr-FPH, respectively, greatly contribute to the prevention of weight loss and colon inflammation in colitic BALB/c mice. T cell proliferation, expansion of TH1 and TH17 cells, and pro-inflammatory cytokines are lower, whereas Treg cells are higher in spleen cell cultures from Phi-treated mice. In addition, therapeutic phr-FPH treatment is able to reduce the expansion of TH17 in splenic cell cultures. Conclusion: The consumption of phenolic and protein compounds extracted from flaxseeds has a protective effect on TNBS-induced colitis, and may be useful in the control of other inflammatory disorders. (AU) | |
| FAPESP's process: | 13/20258-2 - Effects of oral tolerance and tolerogenic dendritic cell administration on the immune response in experimental colitis |
| Grantee: | Patricia Ucelli Simioni |
| Support Opportunities: | Regular Research Grants |