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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Intake of Protein Hydrolysates and Phenolic Fractions Isolated from Flaxseed Ameliorates TNBS-Induced Colitis

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Silva, Fernanda G. D. E. [1, 2] ; Paiatto, Lisiery N. [3, 4] ; Yamada, Aureo T. [5] ; Netto, Flavia M. [1] ; Simioni, Patricia U. [3, 4, 6] ; Tamashiro, Wirla M. S. C. [3]
Total Authors: 6
[1] Univ Estadual Campinas, UNICAMP, Sch Food Engn, Dept Food & Nutr, BR-13083862 Campinas, SP - Brazil
[2] Univ Fed Ouro Preto, Sch Nutr, Dept Food, BR-3540000 Ouro Preto - Brazil
[3] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Genet Evolut Microbiol & Immunol, BR-13083970 Campinas, SP - Brazil
[4] Univ Estadual Paulista, UNESP, Inst Biosci, Dept Biochem & Immunol, BR-13506900 Rio Claro - Brazil
[5] Univ Estadual Campinas, UNICAMP, Inst Biol, Dept Biochem & Tissue Biol, BR-13506970 Campinas, SP - Brazil
[6] Fac Amer FAM, Dept Biomed Sci, BR-13477360 Americana - Brazil
Total Affiliations: 6
Document type: Journal article
Web of Science Citations: 3

Scope: In the attempt to develop new therapeutic treatments for colitis, fractions containing phenolic compound isolate (Phi) and phenolic reduced-flaxseed protein hydrolysate (phr-FPH) from flaxseed are evaluated for their effects on the in vitro production of pro-inflammatory mediators and on the course of experimental colitis. Methods and results: The anti-inflammatory effects of Phi and phr-FPH from flaxseeds are studied in RAW264.7 cells and in trinitrobenzene sulphonic acid (TNBS) colitis model. It is observed that the incubation with Phi or phr-FPH result in lower levels of tumor necrosis factor and nitric oxide in macrophages stimulated with bacterial lipopolysaccharide + interferon-gamma. Prophylactic and therapeutic treatments with Phi and phr-FPH, respectively, greatly contribute to the prevention of weight loss and colon inflammation in colitic BALB/c mice. T cell proliferation, expansion of TH1 and TH17 cells, and pro-inflammatory cytokines are lower, whereas Treg cells are higher in spleen cell cultures from Phi-treated mice. In addition, therapeutic phr-FPH treatment is able to reduce the expansion of TH17 in splenic cell cultures. Conclusion: The consumption of phenolic and protein compounds extracted from flaxseeds has a protective effect on TNBS-induced colitis, and may be useful in the control of other inflammatory disorders. (AU)

FAPESP's process: 13/20258-2 - Effects of oral tolerance and tolerogenic dendritic cell administration on the immune response in experimental colitis
Grantee:Patricia Ucelli Simioni
Support type: Regular Research Grants