Genotypic Resistance to Quinolone and Tetracycline in Salmonella Dublin Strains Isolated from Humans and Animals in Brazil

Autor(es)

Vilela, Felipe Pinheiro ^[1] ; Gomes, Carolina Nogueira ^[2] ; Passaglia, Jaqueline ^[2] ; Rodrigues, Dalia Prazeres ^[3] ; Costa, Renata Garcia ^[3] ; Tiba Casas, Monique Ribeiro ^[4] ; Fernandes, Sueli Aparecida ^[4] ; Falcao, Juliana Pfrimer ^[2] ; Campioni, Fabio ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Av Cafe S-N, Bloco S, Sala 41, BR-14040903 Ribeirao Preto - Brazil

^[3] Fundaco Oswaldo Cruz FIOCRUZ, Rio De Janeiro - Brazil

^[4] Adolfo Lutz Inst, Ctr Bacteriol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MICROBIAL DRUG RESISTANCE; v. 25, n. 2 SEP 15 2018.
• Citações Web of Science: 2

Resumo

Resistance of Salmonella Dublin strains to quinolones and tetracycline has been increasing worldwide. Studies regarding the genotypic resistance traits of strains of this serovar isolated in Brazil are scarce. This study aims to examine the genetic characteristics of Salmonella Dublin strains isolated in Brazil, which are associated with resistance to quinolone and tetracycline. The minimum inhibitory concentrations (MICs) of nalidixic acid, ciprofloxacin, and tetracycline of the 10 strains sensitive and 21 strains resistant to quinolone and tetracycline were determined using Etest.((R)) The mutation profiles of the gyrA, gyrB, parC, and parE genes were accessed by sequencing, while the presence of plasmid-mediated quinolone resistance and tet genes was analyzed by PCR. Quinolone-resistant strains presented the amino acid substitutions Ser96Tyr, Ser96Phe, Asp107Asn, or Asp108Gly on the gyrA gene, and the Ser224Phe and Glu231Asp mutations on the gyrB gene. The qnrA, tet(A), and tet(B) genes were detected in 5, 13, and 6 strains, respectively. Analysis of the MIC values revealed that 1 and 3 strains presented intermediate and resistant MIC profiles to nalidixic acid, respectively; 6 strains presented intermediate MIC profile to ciprofloxacin; and 13 strains presented resistant MIC profile to tetracycline. In the Salmonella Dublin strains studied, quinolone resistance was mainly related to mutation points that led to target alteration in the gyrA and gyrB genes, while tetracycline resistance was associated with the presence of tet(A) and/or tet(B) genes, with the highest resistance levels detected in strains bearing the tet(B) gene. The presence of the aforementioned genotypic resistance traits in Salmonella Dublin strains isolated over 33 years in Brazil indicates that ciprofloxacin or tetracycline therapy against such strains may fail. (AU)

• Processo FAPESP: 17/05756-7 - Análise da invasão celular, sobrevivência em macrófagos e expressão dos genes st313-td, sopE2 e fliC em linhagens de Salmonella Dublin isoladas no Brasil
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 14/13029-0 - Caracterização molecular de linhagens de Campylobacter jejuni e Campylobacter coli de origens diversas
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/10818-6 - Comparação fenotípica e genotípica de linhagens de Salmonella Dublin isoladas de humanos e animais no Brasil
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/25191-3 - Análise fenotípica e sequenciamento do transcriptoma e genoma em linhagens de Salmonella Enteritidis isoladas no período pré e pós-pandemia no Brasil
• Beneficiário: Fábio Campioni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado