Genotypic Resistance to Quinolone and Tetracycline in Salmonella Dublin Strains Isolated from Humans and Animals in Brazil

Author(s)

Vilela, Felipe Pinheiro ^[1] ; Gomes, Carolina Nogueira ^[2] ; Passaglia, Jaqueline ^[2] ; Rodrigues, Dalia Prazeres ^[3] ; Costa, Renata Garcia ^[3] ; Tiba Casas, Monique Ribeiro ^[4] ; Fernandes, Sueli Aparecida ^[4] ; Falcao, Juliana Pfrimer ^[2] ; Campioni, Fabio ^[2]

Affiliation

^[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Ribeirao Preto - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Av Cafe S-N, Bloco S, Sala 41, BR-14040903 Ribeirao Preto - Brazil

^[3] Fundaco Oswaldo Cruz FIOCRUZ, Rio De Janeiro - Brazil

^[4] Adolfo Lutz Inst, Ctr Bacteriol, Sao Paulo - Brazil

• Document type: Journal article
• Source: MICROBIAL DRUG RESISTANCE; v. 25, n. 2 SEP 15 2018.
• Web of Science Citations: 2

Abstract

Resistance of Salmonella Dublin strains to quinolones and tetracycline has been increasing worldwide. Studies regarding the genotypic resistance traits of strains of this serovar isolated in Brazil are scarce. This study aims to examine the genetic characteristics of Salmonella Dublin strains isolated in Brazil, which are associated with resistance to quinolone and tetracycline. The minimum inhibitory concentrations (MICs) of nalidixic acid, ciprofloxacin, and tetracycline of the 10 strains sensitive and 21 strains resistant to quinolone and tetracycline were determined using Etest.((R)) The mutation profiles of the gyrA, gyrB, parC, and parE genes were accessed by sequencing, while the presence of plasmid-mediated quinolone resistance and tet genes was analyzed by PCR. Quinolone-resistant strains presented the amino acid substitutions Ser96Tyr, Ser96Phe, Asp107Asn, or Asp108Gly on the gyrA gene, and the Ser224Phe and Glu231Asp mutations on the gyrB gene. The qnrA, tet(A), and tet(B) genes were detected in 5, 13, and 6 strains, respectively. Analysis of the MIC values revealed that 1 and 3 strains presented intermediate and resistant MIC profiles to nalidixic acid, respectively; 6 strains presented intermediate MIC profile to ciprofloxacin; and 13 strains presented resistant MIC profile to tetracycline. In the Salmonella Dublin strains studied, quinolone resistance was mainly related to mutation points that led to target alteration in the gyrA and gyrB genes, while tetracycline resistance was associated with the presence of tet(A) and/or tet(B) genes, with the highest resistance levels detected in strains bearing the tet(B) gene. The presence of the aforementioned genotypic resistance traits in Salmonella Dublin strains isolated over 33 years in Brazil indicates that ciprofloxacin or tetracycline therapy against such strains may fail. (AU)

• FAPESP's process: 17/05756-7 - Cell invasion, macrophage survival analysis and expression of the genes st313-td, sopE2 and fliC in Salmonella Dublin strains isolated in Brazil
• Grantee: Felipe Pinheiro Vilela
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 14/13029-0 - Molecular characterization of Campylobacter jejuni and Campylobacter coli strains of diverse origins
• Grantee: Juliana Pfrimer Falcão
• Support Opportunities: Regular Research Grants
• FAPESP's process: 15/10818-6 - Phenotypic and genotypic comparison of Salmonella Dublin strains isolated from humans and animals in Brazil
• Grantee: Felipe Pinheiro Vilela
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• FAPESP's process: 13/25191-3 - Phenotypical analysis and transcriptome and genome sequencing in Salmonella Enteritidis strains isolated in the pre and post-pandemic period in Brazil
• Grantee: Fábio Campioni
• Support Opportunities: Scholarships in Brazil - Post-Doctoral