Cell invasion, macrophage survival analysis and expression of the genes st313-td, sopE2 and fliC in Salmonella Dublin strains isolated in Brazil

• Grant number: 17/05756-7
• Support Opportunities: Scholarships in Brazil - Scientific Initiation
• Start date: June 01, 2017
• End date: December 31, 2018
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Juliana Pfrimer Falcão
• Grantee: Felipe Pinheiro Vilela
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil

Abstract

Salmonella Dublin is a serovar strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, sporadically it can be isolated from humans, usually causing serious disease, especially in patients with underlying chronic diseases. Differently of S. Dublin, bloodstream infections caused by other non-typhoidal serovars are not usual. However, in the Subsaarian Africa, non-typhoidal salmonella are among the most isolated pathogens related to bacteremia and meningitis. Salmonella Typhimurium accounts for a significant proportion of these infections and, strains belonging to a specific MultiLocus Sequence Type (MLST), the ST 313, have been related to these cases. A possible virulence marker found in the strains of the ST 313, the st313-td gene, interestingly is not present in the S. Typhimurium strains from other STs. However, it has been reported that this gene has been found in all S. Dublin strains. This project aim to verify the prevalence and expression of the st313-td gene, the expression of the genes sopE2 e fliC, related to invasion and flagella release, respectively, besides the ability to invade epithelial cells and survive in macrophages in Salmonella Dublin strains isolated in Brazil. For this, 115 S. Dublin strains isolated from humans and animals in Brazil will be studied. The st-313td gene will be searched by PCR and 20 strains will be selected for the human epithelial cell invasion assays (CACO-2) and survival in human macrophages (U937). Among these 20 strains, five will be selected to verify the expression of the st313-td, sopE2 and fliC genes. The results will provide data about the frequency and expression of the st313-td gene in S. Dublin strains isolated in Brazil. Moreover, this study will provide additional data about serovar Dublin, which few have studied, regarding the virulence mechanisms that permit it to cause invasive disease in humans and animals. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

VILELA, FELIPE PINHEIRO; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil. Brazilian Journal of Microbiology, v. 51, n. 2, NOV 2019. (17/05756-7, 19/06947-6, 13/25191-3, 16/24716-3)

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; MILLER, DANIELA; LEON, MARIA SANCHEZ; TIBA-CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; RODRIGUES, DANA DOS PRAZERES; COSTA, RENATA GARCIA; et al. Draft Genome Sequences of 112 Salmonella enterica Serovar Dublin Strains Isolated from Humans and Animals in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 24, JUN 2018. (17/05756-7, 16/05817-3, 15/10818-6, 16/24716-3, 13/25191-3)

VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PAZIANI, MARIO HENRIQUE; BRAZ, VANIA SANTOS; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA-CASAS, MONIQUE RIBEIRO; VON ZESKA KRESS, MARCIA REGINA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil. INFECTION GENETICS AND EVOLUTION, v. 80, JUN 2020. (13/25191-3, 17/05756-7, 19/06947-6, 16/24716-3)

VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PASSAGLIA, JAQUELINE; RODRIGUES, DALIA PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Genotypic Resistance to Quinolone and Tetracycline in Salmonella Dublin Strains Isolated from Humans and Animals in Brazil. MICROBIAL DRUG RESISTANCE, v. 25, n. 2, SEP 15 2018. (17/05756-7, 14/13029-0, 15/10818-6, 13/25191-3)

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; MILLER, DANIELA; LEON, MARIA SANCHEZ; TIBA-CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; RODRIGUES, DANA DOS PRAZERES; COSTA, RENATA GARCIA; et al. Draft Genome Sequences of 112 Salmonella enterica Serovar Dublin Strains Isolated from Humans and Animals in Brazil. GENOME ANNOUNCEMENTS, v. 6, n. 24, p. 4-pg., 2018-06-01. (17/05756-7, 15/10818-6, 16/24716-3, 16/05817-3, 13/25191-3)