Phenotypical analysis and transcriptome and genome sequencing in Salmonella Enteritidis strains isolated in the pre and post-pandemic period in Brazil

• Grant number: 13/25191-3
• Support Opportunities: Scholarships in Brazil - Post-Doctoral
• Start date: March 01, 2014
• End date: February 28, 2018
• Field of knowledge: Biological Sciences - Microbiology - Biology and Physiology of Microorganisms
• Principal Investigator: Juliana Pfrimer Falcão
• Grantee: Fábio Campioni
• Host Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Associated scholarship(s): 16/05817-3 - Whole genome sequencing and bioinformatics analysis of Salmonella enteritidis strains isolated in the pre and post-pandemic period in Brazil, BE.EP.PD

Abstract

The disease caused by Salmonella is one of the major health problem worldwide in terms of morbid and mortality. Among the Salmonella enterica serovars, the Enteritidis (S. Enteritidis) comprises strains that have specialized ability to colonize the reproductive tissue of poultry and contaminate internal components of eggs, which allowed S. Enteritidis to become the most common isolated serovar of Salmonella spp. causing human disease. Although the reasons that led to this emergence are uncertain, it is believed that an epidemical and more virulent clone has been inserted in the country through the exchange of chickens with other countries. The aim of this project is to comparatively analyze by phenotypical tests, transcriptome sequencing and genome sequencing Salmonella Enteritidis strains isolated in the pre and post-pandemic period in Brazil. For this, it will be studied 48 S. Enteritidis strains, being 24 strains isolated in the pre-pandemic period (1968-1992) and 24 strains isolated in the post-pandemic period (1993-2013) in several places of Brazil. The strains isolated in the pre and post-pandemic period will be compared regarding the invasion to human and chicken's epithelial cells, survival capability against phagocytosis by human and chicken macrophages, survival to the acid and oxidative stress and ability to grow in the egg albumen. The comparison of the expressed genes in each of the phenotypical tests described above will be performed for one strain of Salmonella Enteritidis isolated in the pre-pandemic period and one isolated in the post pandemic period that will present significative difference in the majority of the phenotypical tests, using transcriptome sequencing. Data of the differential expression of genes will be validated by RT-PCR. Moreover, the two selected strains will have their genome sequenced and the minimal lethal dose (DL50) will be determined. Together, the results should contribute for a better characterization of S. Enteritidis, as well as, it will provide information about the differences between strains isolated in the pre and post pandemic period in the country, which could collaborate to elucidate the reasons of the beginning of the pandemic in Brazil, as well as, may provide additional data to other studies about the emergence of the serovar Enteritidis as the most prevalent one in several countries worldwide.

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

MARTINS IMORI, PRISCILLA FERNANDA; CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; LEON, MARIA SANCHEZ; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of Yersinia frederiksenii, Yersinia intermedia, and Yersinia kristensenii Strains from Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 5, n. 32, AUG 2017. (16/05817-3, 16/24716-3, 13/25191-3)

CAMPIONI, FABIO; GOMES, CAROLINA NOGUEIRA; RODRIGUES, DALIA DOS PRAZERES; BERGAMINI, ALZIRA MARIA MORATO; FALCAO, JULIANA PFRIMER. henotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazi. Brazilian Journal of Microbiology, v. 52, n. 1, SI, OCT 2020. (16/24716-3, 13/25191-3)

VILELA, F. P.; FRAZAO, M. R.; RODRIGUES, D. P.; COSTA, R. G.; CASAS, M. R. T.; FERNANDES, S. A.; FALCAO, J. P.; CAMPIONI, F.. Genetic diversity, anti-microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil. ZOONOSES AND PUBLIC HEALTH, v. 65, n. 1, p. E34-E43, FEB 2018. (14/13029-0, 15/10818-6, 13/25191-3)

CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; JANIES, DANIEL A.; MORATO BERGAMINI, ALZIRA MARIA; RODRIGUES, DALIA DOS PRAZERES; STONES, ROBERT; BROWN, ERIC; ALLARD, MARC W.; FALCAO, JULIANA PFRIMER. Changing of the Genomic Pattern of Salmonella Enteritidis Strains Isolated in Brazil Over a 48 year-period revealed by Whole Genome SNP Analyses. SCIENTIFIC REPORTS, v. 8, JUL 11 2018. (14/13029-0, 16/05817-3, 13/25191-3)

VILELA, FELIPE PINHEIRO; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. High similarity and high frequency of virulence genes among Salmonella Dublin strains isolated over a 33-year period in Brazil. Brazilian Journal of Microbiology, v. 51, n. 2, NOV 2019. (17/05756-7, 19/06947-6, 13/25191-3, 16/24716-3)

CAMPIONI, FABIO; GOMES, CAROLINA N.; BERGAMINI, ALZIRA M. M.; RODRIGUES, DALIA P.; FALCAO, JULIANA P.. Partial Correlation Between Phenotypic and Genotypic Antimicrobial Resistance of Salmonella enterica Serovar Enteritidis Strains from Brazil. MICROBIAL DRUG RESISTANCE, v. 26, n. 12, APR 2020. (13/25191-3, 16/24716-3)

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA-CASAS, MONIQUE RIBEIRO; YIN, LANLAN; ALLARD, MARC; FALCAO, JULIANA PFRIMER. Whole genome sequencing analyses revealed that Salmonella enterica serovar Dublin strains from Brazil belonged to two predominant clades. SCIENTIFIC REPORTS, v. 12, n. 1, p. 6-pg., 2022-06-22. (16/05817-3, 16/24716-3, 13/25191-3, 19/19338-8)

CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; LEON, MARIA SANCHEZ; MORATO BERGAMINI, ALZIRA MARIA; RODRIGUES, DALIA DOS PRAZERES; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 256 Salmonella enterica subsp. enterica Serovar Enteritidis Strains Isolated from Humans, Food, Chickens, and Farm Environments in Brazil. GENOME ANNOUNCEMENTS, v. 5, n. 28, p. 6-pg., 2017-07-01. (12/19132-1, 13/25191-3, 16/05817-3)

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; MILLER, DANIELA; LEON, MARIA SANCHEZ; TIBA-CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; RODRIGUES, DANA DOS PRAZERES; COSTA, RENATA GARCIA; et al. Draft Genome Sequences of 112 Salmonella enterica Serovar Dublin Strains Isolated from Humans and Animals in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 6, n. 24, JUN 2018. (17/05756-7, 16/05817-3, 15/10818-6, 16/24716-3, 13/25191-3)

CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; LEON, MARIA SANCHEZ; MORATO BERGAMINI, ALZIRA MARIA; RODRIGUES, DALIA DOS PRAZERES; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of 256 Salmonella enterica subsp. enterica Serovar Enteritidis Strains Isolated from Humans, Food, Chickens, and Farm Environments in Brazil. MICROBIOLOGY RESOURCE ANNOUNCEMENTS, v. 5, n. 28, JUL 2017. (16/05817-3, 12/19132-1, 13/25191-3)

VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PAZIANI, MARIO HENRIQUE; BRAZ, VANIA SANTOS; RODRIGUES, DALIA DOS PRAZERES; COSTA, RENATA GARCIA; TIBA-CASAS, MONIQUE RIBEIRO; VON ZESKA KRESS, MARCIA REGINA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Virulence traits and expression of bstA, fliC and sopE2 in Salmonella Dublin strains isolated from humans and animals in Brazil. INFECTION GENETICS AND EVOLUTION, v. 80, JUN 2020. (13/25191-3, 17/05756-7, 19/06947-6, 16/24716-3)

VILELA, FELIPE PINHEIRO; GOMES, CAROLINA NOGUEIRA; PASSAGLIA, JAQUELINE; RODRIGUES, DALIA PRAZERES; COSTA, RENATA GARCIA; TIBA CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; FALCAO, JULIANA PFRIMER; CAMPIONI, FABIO. Genotypic Resistance to Quinolone and Tetracycline in Salmonella Dublin Strains Isolated from Humans and Animals in Brazil. MICROBIAL DRUG RESISTANCE, v. 25, n. 2, SEP 15 2018. (17/05756-7, 14/13029-0, 15/10818-6, 13/25191-3)

MARTINS IMORI, PRISCILLA FERNANDA; CAMPIONI, FABIO; CAO, GUOJIE; KASTANIS, GEORGE; LEON, MARIA SANCHEZ; ALLARD, MARC WILLIAM; FALCAO, JULIANA PFRIMER. Draft Genome Sequences of Yersinia frederiksenii, Yersinia intermedia, and Yersinia kristensenii Strains from Brazil. GENOME ANNOUNCEMENTS, v. 5, n. 32, p. 2-pg., 2017-08-01. (13/25191-3, 16/24716-3, 16/05817-3)

CAMPIONI, FABIO; GOMES, CAROLINA NOGUEIRA; RODRIGUES, DALIA DOS PRAZERES; BERGAMINI, ALZIRA MARIA MORATO; FALCAO, JULIANA PFRIMER. Phenotypic analyses of Salmonella enterica serovar Enteritidis strains isolated in the pre- and post-epidemic period in Brazil. Brazilian Journal of Microbiology, v. 52, n. 1, p. 11-pg., 2020-10-26. (13/25191-3, 16/24716-3)

CAMPIONI, FABIO; VILELA, FELIPE PINHEIRO; CAO, GUOJIE; KASTANIS, GEORGE; MILLER, DANIELA; LEON, MARIA SANCHEZ; TIBA-CASAS, MONIQUE RIBEIRO; FERNANDES, SUELI APARECIDA; RODRIGUES, DANA DOS PRAZERES; COSTA, RENATA GARCIA; et al. Draft Genome Sequences of 112 Salmonella enterica Serovar Dublin Strains Isolated from Humans and Animals in Brazil. GENOME ANNOUNCEMENTS, v. 6, n. 24, p. 4-pg., 2018-06-01. (17/05756-7, 15/10818-6, 16/24716-3, 16/05817-3, 13/25191-3)