Salmonella Gallinarum (SG): 1. Sequencing of the genome of the attenuate strain SG "cobS" cbiA and assessment of its gene expression in macrophages. 2. Comparative analysis of SG and S. Pullorum genomes. 3. Assessment of virulence of motile SG strains

• Grant number: 11/23483-1
• Support type: Regular Research Grants
• Duration: June 01, 2012 - May 31, 2014
• Field of knowledge: Agronomical Sciences - Veterinary Medicine - Animal Pathology
• Principal researcher: Angelo Berchieri Junior
• Grantee: Angelo Berchieri Junior
• Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil

Abstract

This project is toward to study aspects of the cellular biology of Salmonella serotypes Enteritidis (SE) and Gallinarum biovars Gallinarum (SG) and Pullorum (SP) and the prevention of the infection of chickens by SE and SG as well. We have produced an attenuated strains of SG (SG "cobS"cbiA) that could be used to protect birds against fowl typhoid and the infection by SE. We are going to sequencing this strain to identify the altered region and also, it will be inoculated in avian macrophages cells to analysis the gene expression by the microorganism and the macrophages. Another study will be carried out to compare the genomic material of the biovars SG e SP. Although they are very closed microorganisms they provoke different diseases. The last approach will be made with a strain of SG containing flagella. There are some suggestions that the absence of flagella in SG e SP impede the immune system to control the infection. Therefore, making flagellated mutants of SG we will check that. (AU)

Scientific publications

(5)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

DEL PILAR CELIS-ESTUPINAN, ANNY LUCIA; ALVES BATISTA, DIEGO FELIPE; CARDOZO, MARITA VEDOVELLI; SECUNDO DE SOUZA, ANDREI ITAJAHY; RODRIGUES ALVES, LUCAS BOCCHINI; DE ALMEIDA, ADRIANA MARIA; BARROW, PAUL ANDREW; BERCHIERI, JR., ANGELO; DE FREITAS NETO, OLIVEIRO CAETANO. Further investigations on the epidemiology of fowl typhoid in Brazil. AVIAN PATHOLOGY, v. 46, n. 4, p. 416-425, 2017. Web of Science Citations: 2.

LOPES, P. D.; FREITAS NETO, O. C.; BATISTA, D. F. A.; DENADAI, J.; ALARCON, M. F. F.; ALMEIDA, A. M.; VASCONCELOS, R. O.; SETTA, A.; BARROW, P. A.; BERCHIERI JUNIOR, A. Experimental infection of chickens by a flagellated motile strain of Salmonella enterica serovar Gallinarum biovar Gallinarum. VETERINARY JOURNAL, v. 214, p. 40-46, AUG 2016. Web of Science Citations: 4.

ALVES BATISTA, DIEGO FELIPE; DE FREITAS NETO, OLIVEIRO CAETANO; DE ALMEIDA, ADRIANA MARIA; BARROW, PAUL ANDREW; BARBOSA, FERNANDA DE OLIVEIRA; BERCHIERI JUNIOR, ANGELO. Molecular identification of Salmonella enterica subsp enterica serovar Gallinarum biovars Gallinarum and Pullorum by a duplex PCR assay. JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION, v. 28, n. 4, p. 419-422, JUL 2016. Web of Science Citations: 4.

SECUNDO DE SOUZA, ANDREI ITAJAHY; DE FREITAS NETO, OLIVEIRO CAETANO; ALVES BATISTA, DIEGO FELIPE; DEL PILAR CELIS ESTUPINAN, ANNY LUCIA; DE ALMEIDA, ADRIANA MARIA; BARROW, PAUL ANDREW; BERCHIERI, ANGELO. ERIC-PCR genotyping of field isolates of Salmonella enterica subsp enterica serovar Gallinarum biovars Gallinarum and Pullorum. AVIAN PATHOLOGY, v. 44, n. 6, p. 475-479, NOV 2 2015. Web of Science Citations: 4.

ALVES BATISTA, DIEGO FELIPE; FREITAS NETO, OLIVEIRO CAETANO; BARROW, PAUL ANDREW; DE OLIVEIRA, MARCOS TULIO; ALMEIDA, ADRIANA MARIA; FERRAUDO, ANTONIO SERGIO; BERCHIERI, JR., ANGELO. Identification and characterization of regions of difference between the Salmonella Gallinarum biovar Gallinarum and the Salmonella Gallinarum biovar Pullorum genomes. INFECTION GENETICS AND EVOLUTION, v. 30, p. 74-81, MAR 2015. Web of Science Citations: 8.

Filed patent(s) as a result of this research project