Pressurized liquid extraction of flavanols and alkaloids from cocoa bean shell using ethanol as solvent

Autor(es)

Okiyama, Dayane C. G. ^[1] ; Soares, Ingrid D. ^[1] ; Cuevas, Maite S. ^[1] ; Crevelin, Eduardo J. ^[2] ; Moraes, Luiz A. B. ^[2] ; Melo, Mariza P. ^[3] ; Oliveira, Alessandra L. ^[4] ; Rodrigues, Christianne E. C. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Food Engn, Separat Engn Lab LES, BR-13635900 Pirassununga, SP - Brazil

^[2] Univ Sao Paulo, Dept Chem, Mass Spectrometry Lab, BR-14040901 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Dept Basic Sci, Biol Chem Lab LQB, BR-13635900 Pirassununga, SP - Brazil

^[4] Univ Sao Paulo, Dept Food Engn, High Pressure Lab & Nat Prod LTAPPN, BR-13635900 Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 114, p. 20-29, DEC 2018.
• Citações Web of Science: 7

Resumo

Cocoa shell (CS) is a co-product of the cocoa industry used mainly as fuel for boilers but with secondary applications as fertilizer and in animal feed. Although it is known that this material is rich in flavanols and alkaloids, to date, a study has not been conducted that has quantitatively identified these compounds in CS. Thus, the aim of this work was to characterize CS in terms of its composition, regarding catechin, epicatechin, procyanidin B2, caffeine and theobromine, and to evaluate the extraction kinetics of the total flavanols using pressurized liquid extraction (PLE) with absolute ethanol. For the determination of the extraction kinetic data, the DMAC method was used, while each compound was quantified using a UPLC-MS/MS analysis. The major compounds found were theobromine and epicatechin (mean values of 9.89 and 3.5 mg/g CS, respectively). PLE proved to be quite effective; the flavanols extraction yield was enhanced by increasing the temperature and extraction time however, high extraction times and temperatures degraded the procyanidins B2. Peleg's model applied to extraction data description provided a reasonable agreement with the experimental results, which allows their application in modeling and optimization of solid-liquid extraction of the total flavanols from cocoa bean shell. (AU)

• Processo FAPESP: 14/09446-4 - Desenvolvimento de processo de extração de óleos vegetais utilizando solventes alcoólicos: avaliação da composição do óleo e de alterações na fração protéica
• Beneficiário: Christianne Elisabete da Costa Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/03579-5 - Reaproveitamento da Casca da Amêndoa de Cacau para Extração de Gordura Utilizando Solventes Alcoólicos
• Beneficiário: Dayane Cristina Gomes Okiyama
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 17/20840-4 - Valorização de coprodutos agroindustriais através da obtenção de óleos vegetais e compostos minoritários via extração com solventes
• Beneficiário: Christianne Elisabete da Costa Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/21252-0 - Equilíbrio e processos de produção de biocombustíveis e bioprodutos
• Beneficiário: Antonio José de Almeida Meirelles
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/15865-5 - Extração alcoólica de gordura e biocompostos de cascas de amêndoas de cacau
• Beneficiário: Ingrid Denardi Soares
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica