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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Influence of oxidative damage to proteins on meat tenderness using a proteomics approach

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Autor(es):
Malheiros, Jessica Moraes [1] ; Braga, Camila Pereira [2] ; Grove, Ryan Albert [2] ; Ribeiro, Felipe Azevedo [3] ; Calkins, Chris Richard [3] ; Adamec, Jiri [2] ; Loyola Chardulo, Luis Artur [4]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, Coll Agr & Vet Sci, Dept Anim Sci, Jaboticabal, SP - Brazil
[2] Univ Nebraska, Dept Biochem, Redox Biol Ctr, Lincoln, NE - USA
[3] Univ Nebraska, Dept Anim Sci, Lincoln, NE - USA
[4] Sao Paulo State Univ, Coll Vet & Anim Sci, Dept Breeding & Nutr Anim, Botucatu, SP - Brazil
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: MEAT SCIENCE; v. 148, p. 64-71, FEB 2019.
Citações Web of Science: 5
Resumo

The objective of this study was to evaluate the association between oxidative damage to proteins (represented by protein carbonylation) and beef tenderness. Three experimental groups were selected by shear force (SF): tender (38.2 +/- 2.9 N), intermediate (51.9 +/- 6.8 N), and tough meat (74.5 +/- 7.8 N). Two-dimensional electrophoresis with hydrazide fluorophore derivatization was used. The structural proteins actin (ACTA1), myosin (MYL1 and MYL3), desmin (DES) and troponin T (TNNT1 and TNNT3), antioxidant proteins (PRDX1, PRDX2 and PARK7) and heat shock proteins (HSPB1, CRYAB and HSPB6) showed an increase in the oxidative damage in tender meat when compared to the intermediate and tough meat (P < .05). Decrease in oxidative damage of the metabolic enzymes (TPI1, GAPDH and ENO3) were observed in tender meat group (P < .05). The present results suggest that oxidation act on the proteins of different metabolic pathways and consequently affect meat tenderness in Angus crossbred cattle. (AU)

Processo FAPESP: 15/13021-1 - Abordagem proteômica metalômica e de expressão de proteínas estruturais, proteolíticas e reguladoras do tecido muscular esquelético no post-mortem e suas relações com o crescimento e a maciez da carne em animais da raça Nelore (Bos indicus)
Beneficiário:Luis Artur Loyola Chardulo
Linha de fomento: Auxílio à Pesquisa - Regular