MiRNAs differentially expressed in skeletal muscle of animals with divergent estimated breeding values for beef tenderness

Autor(es)

Kappeler, Berna I. G. ^[1] ; Regitano, Luciana C. A. ^[2] ; Poleti, Mirele D. ^[3] ; Cesar, Aline S. M. ^[1] ; Moreira, Gabriel C. M. ^[1] ; Gasparin, Gustavo ^[1] ; Coutinho, Luiz L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz De Queiroz Coll Agr, Dept Anim Sci, BR-13418900 Piracicaba, SP - Brazil

^[2] Embrapa Pecuaria Sudeste, BR-13560970 Sao Carlos, SP - Brazil

^[3] Univ Sao Paulo, Fac Anim Sci & Food Engn, Dept Vet Med, BR-13635900 Pirassununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: BMC MOLECULAR BIOLOGY; v. 20, JAN 3 2019.
• Citações Web of Science: 2

Resumo

BackgroundMicroRNAs (miRNAs) are small noncoding RNAs of approximately 22 nucleotides, highly conserved among species, which modulate gene expression by cleaving messenger RNA target or inhibiting translation. MiRNAs are involved in the regulation of many processes including cell proliferation, differentiation, neurogenesis, angiogenesis, and apoptosis. Beef tenderness is an organoleptic characteristic of great influence in the acceptance of meat by consumers. Previous studies have shown that collagen level, marbling, apoptosis and proteolysis are among the many factors that affect beef tenderness. Considering that miRNAs can modulate gene expression, this study was designed to identify differentially expressed miRNAs that could be modulating biological processes involved with beef tenderness.ResultsDeep sequence analysis of miRNA libraries from longissimus thoracis muscle allowed the identification of 42 novel and 308 known miRNAs. Among the known miRNAs, seven were specifically expressed in skeletal muscle. Differential expression analysis between animals with high (H) and low (L) estimated breeding values for shear force (EBVSF) revealed bta-mir-182 and bta-mir-183 are up-regulated (q value<0.05) in animals with L EBVSF, and bta-mir-338 is up-regulated in animals with H EBVSF. The number of bovine predicted targets for bta-mir-182, bta-mir-183 and bta-mir-338 were 811, 281 and 222, respectively, which correspond to 1204 unique target genes. Among these, four of them, MEF2C, MAP3K2, MTDH and TNRC6B were common targets of the three differentially expressed miRNAs. The functional analysis identified important pathways related to tenderness such as apoptosis and the calpain-calpastatin system.ConclusionThe results obtained indicate the importance of miRNAs in the regulatory mechanisms that influence muscle proteolysis and meat tenderness and contribute to our better understanding of the role of miRNAs in biological processes associated with beef tenderness. (AU)

• Processo FAPESP: 12/23638-8 - Bases moleculares da qualidade da carne em bovinos da raça Nelore
• Beneficiário: Luciana Correia de Almeida Regitano
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/21017-9 - Estudo de proteínas associadas a fenótipos de qualidade da carne em bovinos da raça Nelore
• Beneficiário: Mirele Daiana Poleti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/00943-5 - Identificação de microRNAs envolvidos com a maciez da carne em bovinos da raça Nelore
• Beneficiário: Berna Ines Gimenez Kappeler
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/11871-5 - Identificação de eQTLs e estudo de regiões genômicas associadas à deposição e composição da gordura intramuscular em animais da raça Nelore
• Beneficiário: Aline Silva Mello Cesar
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado