RNA sequencing differential gene expression analysis of isolated perfused bovine udders experimentally inoculated with Streptococcus agalactiae

Autor(es)

Sbardella, A. P. ^[1] ; Weller, M. M. D. C. A. ^[2] ; Fonseca, I ^[2] ; Stafuzza, N. B. ^[1] ; Bernardes, P. A. ^[1] ; Silva, F. F. E. ^[3] ; da Silva, M. V. G. B. ^[2] ; Martins, M. F. ^[2] ; Munari, D. P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista UNESP FCAV, Dept Ciencias Exatas, BR-14884900 Jaboticabal - Brazil

^[2] Empresa Brasileira Pesquisa Agr, Embrapa Gado Leite, BR-36038330 Juiz De Fora - Brazil

^[3] Univ Fed Vicosa, Dept Zootecnia, BR-36570000 Vicosa, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF DAIRY SCIENCE; v. 102, n. 2, p. 1761-1767, FEB 2019.
• Citações Web of Science: 1

Resumo

The aim of this study was to elucidate the differential gene expression in the RNA sequencing transcriptome of isolated perfused udders collected from 4 slaughtered Holstein x Zebu crossbred dairy cows experimentally inoculated with Streptococcus agalactiae. We studied 3 different statistical tools (edgeR, baySeq, and Cuffdiff 2). In summary, 2 quarters of each udder were experimentally inoculated with Strep. agalactiae and the other 2 were used as a control. Mammary tissue biopsies were collected at times 0 and 3 h after infection. The total RNA was extracted and sequenced on an Illumina HiSeq 2000 (Illumiria Inc., San Diego, CA). Transcripts were assembled from the reads aligned to the bovine UMD 3.1 reference genome, and the statistical analyses were performed using the previously mentioned tools (edgeR, baySeq, and Cuffdiff 2). Finally, the identified genes were submitted to pathway enrichment analysis. A total of 1,756, 1,161, and 3,389 genes with differential gene expression were identified when using edgeR, baySeq, and Cuffdiff 2, respectively. A total of 122 genes were identified by the overlapping of the 3 methods; however, only the platelet activation presented a significantly enriched pathway. From the results, we suggest the FCER1G, GNAI2, ORAI1, and VASP genes shared among the 3 methods in this pathway for posterior biological validation. (AU)

• Processo FAPESP: 16/22940-3 - Imputação, desequilíbrio de ligação, blocos de haplótipos e endogamia em bovinos Nelore
• Beneficiário: Priscila Arrigucci Bernardes
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 15/25096-6 - Imputação, desequilíbrio de ligação, blocos de haplótipos e endogamia em bovinos Nelore
• Beneficiário: Priscila Arrigucci Bernardes
• Modalidade de apoio: Bolsas no Brasil - Doutorado