Molecular matching of red blood cells is superior to serological matching in sickle cell disease patients

Autor(es)

Daiane Cobianchi da Costa ^[1] ; Jordão Pellegrino Jr ^[2] ; Gláucia Andréia Soares Guelsin ^[3] ; Karina Antero Rosa Ribeiro ^[4] ; Simone Cristina Olenscki Gilli ^[5] ; Lilian Castilho ^[6]

Afiliação do(s) autor(es)

^[1] Universidade Estadual de Campinas - Brasil

^[2] Universidade Estadual de Campinas - Brasil

^[3] Universidade Estadual de Campinas - Brasil

^[4] Universidade Estadual de Campinas - Brasil

^[5] Universidade Estadual de Campinas - Brasil

^[6] Universidade Estadual de Campinas - Brasil

• Tipo de documento: Artigo Científico
• Fonte: Revista Brasileira de Hematologia e Hemoterapia; v. 35, n. 1, p. 35-38, 2013-00-00.

Resumo

OBJECTIVE: To evaluate the usefulness of DNA methods to provide a means to precisely genotypically match donor blood units for the antigen-negative type of 35 sickle cell disease patients<. METHODS: Red blood cell units were investigated for ABO, D, C, c, E, e, K, Fyª, Fy b, Jkª, Jk b, S, s, Diª and RH variants by performing a molecular array (Human Erythrocyte Antigen BeadChipTM, BioArray Solutions), polymerase chain reaction followed by restriction fragment length polymorphism analysis and sequencing of patient samples and donor units that had been serologically matched based on the ABO, Rh and K phenotypes and the presence of antibodies. RESULTS: Matches for 21 of 35 sickle cell disease patients presented discrepancies or mismatches for multiple antigens between the genotype profile and the antigen profile of their serologically-matched blood units. The main discrepancies or mismatches occurred in the RH, FY, JK and MNS systems. Eight Rh alloimmunized patients presented RHD and RHCE variants that had not been serologically identified. According to these results better matches were found for the patients with genotyped units and the patients benefited as shown by better in vivo red blood cell survival. CONCLUSION: Molecular matching is superior to serological matching in sickle cell disease patients, decreasing the risk of transfusion reactions, especially delayed transfusion reactions to existing alloantibodies and preventing alloimmunization. (AU)

• Processo FAPESP: 08/07544-8 - Genotipagem de grupos sanguíneos no suporte transfusional de sangue compatível para pacientes com anemia falciforme
• Beneficiário: Daiane Cobianchi da Costa
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 09/05924-0 - Análise de polimorfismos e expressão de antígenos de grupos sanguíneos: implicações clínicas
• Beneficiário: Lilian Maria de Castilho
• Modalidade de apoio: Auxílio à Pesquisa - Regular