Molecular matching of red blood cells is superior to serological matching in sickle cell disease patients

Author(s)

Daiane Cobianchi da Costa ^[1] ; Jordão Pellegrino Jr ^[2] ; Gláucia Andréia Soares Guelsin ^[3] ; Karina Antero Rosa Ribeiro ^[4] ; Simone Cristina Olenscki Gilli ^[5] ; Lilian Castilho ^[6]

Affiliation

^[1] Universidade Estadual de Campinas - Brasil

^[2] Universidade Estadual de Campinas - Brasil

^[3] Universidade Estadual de Campinas - Brasil

^[4] Universidade Estadual de Campinas - Brasil

^[5] Universidade Estadual de Campinas - Brasil

^[6] Universidade Estadual de Campinas - Brasil

• Document type: Journal article
• Source: Revista Brasileira de Hematologia e Hemoterapia; v. 35, n. 1, p. 35-38, 2013-00-00.

Abstract

OBJECTIVE: To evaluate the usefulness of DNA methods to provide a means to precisely genotypically match donor blood units for the antigen-negative type of 35 sickle cell disease patients<. METHODS: Red blood cell units were investigated for ABO, D, C, c, E, e, K, Fyª, Fy b, Jkª, Jk b, S, s, Diª and RH variants by performing a molecular array (Human Erythrocyte Antigen BeadChipTM, BioArray Solutions), polymerase chain reaction followed by restriction fragment length polymorphism analysis and sequencing of patient samples and donor units that had been serologically matched based on the ABO, Rh and K phenotypes and the presence of antibodies. RESULTS: Matches for 21 of 35 sickle cell disease patients presented discrepancies or mismatches for multiple antigens between the genotype profile and the antigen profile of their serologically-matched blood units. The main discrepancies or mismatches occurred in the RH, FY, JK and MNS systems. Eight Rh alloimmunized patients presented RHD and RHCE variants that had not been serologically identified. According to these results better matches were found for the patients with genotyped units and the patients benefited as shown by better in vivo red blood cell survival. CONCLUSION: Molecular matching is superior to serological matching in sickle cell disease patients, decreasing the risk of transfusion reactions, especially delayed transfusion reactions to existing alloantibodies and preventing alloimmunization. (AU)

• FAPESP's process: 08/07544-8 - BLOOD GROUP GENOTYPING CAN CONTRIBUTE TO THE TRANSFUSION SUPPORT WITH ANTIGEN-MATCHED BLOOD IN SICKLE CELL DISEASE PATIENTS
• Grantee: Daiane Cobianchi da Costa
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 09/05924-0 - Analyses of blood group polymorphisms and antigen expression: clinical implications
• Grantee: Lilian Maria de Castilho
• Support Opportunities: Regular Research Grants