Aluminum-induced high IAA concentration may explain the Al susceptibility in Citrus limonia

Autor(es)

Silva, Carolina M. S. ^[1] ; Cavalheiro, Mariana F. ^[1] ; Bressan, Anna C. G. ^[1] ; Carvalho, Brenda M. O. ^[1] ; Banhos, Otavia F. A. A. ^[1] ; Purgatto, Eduardo ^[2] ; Harakava, Ricardo ^[3] ; Tanaka, Francisco A. O. ^[4] ; Habermann, Gustavo ^[5]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Inst Biociencias, Dept Bot, Programa Posgrad Ciencias Biol Biol Vegetal, UNESP, Av 24-A, 1515, BR-13506900 Rio Claro, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Alimentos & Nutr Expt, NAPAN, FoRC Food Res Ctr, Av Prof Lineu Prestes 580, Bl 14, BR-05508000 Sao Paulo, SP - Brazil

^[3] Ctr P&D Sanidade Vegetal, Lab Bioquim Fitopatol, Inst Biol, Av Conselheiro R Alves 1252, BR-04014002 Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Escola Super Agr Luiz de Queiroz, Dept Fitopatol & Nematol, Av Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

^[5] Univ Estadual Paulista, UNESP, Inst Biociencias, Dept Bot, Av 24-A, 1515, BR-13506900 Rio Claro, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Plant Growth Regulation; v. 87, n. 1, p. 123-137, JAN 2019.
• Citações Web of Science: 1

Resumo

In acidic soils (pH < 5.0), aluminum (Al) occurs as Al3+, which is phytotoxic and reduces the root growth by hormonal imbalance and/or cell wall rigidity. However, the explanations for the decrease in root growth are not clear. A 60-day study was held with `Rangpur' lime (Citrus limonia) plants grown in nutrient solution containing 0 and 1480 A mu M Al. We measured plant biometric data and used root apices to analyze auxin (IAA), the expression of some Al-responsive genes that had been differentially expressed in a transcriptome analysis and anatomical profiles. We found up-regulated expression of multidrug and toxic compound exudation (Cl-MATE channel), citrate synthase (Cl-CS) and pectin methylesterase inhibitor (Cl-PMEI) genes, but while Cl-PMEI expression was increased after 7 days, Cl-CS and Cl-MATE were up-regulated only after 60 days, suggesting that the timing of these events was ineffective against Al. These results could be associated with the Al-induced decrease in root growth and anatomical root damage. In addition, genes related to IAA cell transport were not differentially expressed in the transcriptome analysis. Therefore, high IAA and up-regulation of auxin-related small RNAs suggest Al-induced high IAA concentration rather than Al-induced disruption in IAA distribution in root cells of this species. (AU)

• Processo FAPESP: 16/14216-3 - Os mecanismos de tolerância ao alumínio em Styrax camporum envolvem síntese e secreção de ácidos orgânicos?
• Beneficiário: Brenda Mistral de Oliveira Carvalho
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 13/11370-3 - Expressão gênica em mecanismos de tolerância ao Al3+ comparando espécie do Cerrado (Qualea grandiflora) e limoeiro (Citrus limonia cv. 'Cravo')
• Beneficiário: Carolina de Marchi Santiago da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 13/07914-8 - FoRC - Centro de Pesquisa em Alimentos
• Beneficiário: Bernadette Dora Gombossy de Melo Franco
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
• Processo FAPESP: 14/14386-0 - Referência indireta dos efeitos fisiológicos do Al por meio de técnicas de microscopia em espécies nativas do cerrado
• Beneficiário: Anna Carolina Gressler Bressan
• Modalidade de apoio: Bolsas no Brasil - Doutorado