Effect of periodontal treatment on Aggregatibacter actinomycetemcomitans colonization and serum IgG levels against A-actinomycetemcomitans serotypes and Omp29 of aggressive periodontitis patients

Autor(es)

Rebeis, Estela Sanches ^[1] ; Albuquerque-Souza, Emmanuel ^[1] ; da Silva, Maike Paulino ^[2] ; Giudicissi, Marcela ^[1] ; Mayer, Marcia P. A. ^{[2, 1]} ; Saraiva, Luciana ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Dent, Dept Stomatol, Div Periodont, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Microbiol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ORAL DISEASES; v. 25, n. 2, p. 569-579, MAR 2019.
• Citações Web of Science: 1

Resumo

Objective To evaluate the effect of the periodontal treatment on Aggregatibacter actinomycetemcomitans JP2 clone, and the IgG serum levels against its outer membrane protein (Omp29) and A. actinomycetemcomitans serotypes in aggressive periodontitis (AgP). Subjects and Methods Seventeen patients with generalized (GAgP), 10 with localized (LAgP), and 10 healthy controls were included. AgP participants were submitted to periodontal treatment-scaling and root planing plus antibiotics (SRP+A). Periodontal parameters, for example, probing depth (PD) and clinical attachment loss (CAL), were evaluated at baseline and at 1-year. Serum IgG against Omp29 and serotypes a, b, and c were determined by ELISA. The levels of A. actinomycetemcomitans JP2 clone were determined in subgingival biofilm samples by qPCR. Results Periodontal treatment resulted in significant reductions of PD, CAL, and IgG levels against Omp29, serotypes b, and c. After therapy, IgG levels against A. actinomycetemcomitans serotypes, as well as the levels of the JP2 clone in AgP, became similar to controls. The reduction in JP2 clone count was correlated with a reduction of PD and IgG response against Omp29. Conclusion Scaling and root planing plus antibiotics decreased IgG levels response against Omp29 and A. actinomycetemcomitans serotypes involved in the disease (b and c), while the serum response increased against tne commensal serotype (a), similar to what occurs in periodontally healthy individuals. (AU)

• Processo FAPESP: 13/26381-0 - Análise de citocinas (no fluido gengival e no soro) e de patógenos periodontais (Aggregatibacter actinomycetemcomitans, Porphyromonas Gingivalis, Tannerella forsythia e Treponema denticola) de pacientes com periodontite agressiva. estudo longitudinal
• Beneficiário: Luciana Saraiva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 12/05911-9 - Aggregatibacter actinomycetemcomitans: análise da virulência e do transcriptoma de cepas de linhagens JP2-like e não- JP2 like sorotipo b na indução de peri-implantite experimental em animal
• Beneficiário: Maike Paulino da Silva
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 10/16162-1 - Análise da resposta de IgG sérica contra a OMP29 e célula total de A. actinomycetemcomitans de diferentes sorotipos e contra célula total de P. gingivalis e T. forsythia de pacientes portadores de periodontite agressiva
• Beneficiário: Luciana Saraiva
• Modalidade de apoio: Auxílio à Pesquisa - Regular