Interesterified Fats Induce Deleterious Effects on Adipose Tissue and Liver in LDLr-KO Mice

Autor(es):Mostrar menos -

Ferrari Lavrador, Maria Silvia ^[1] ; Afonso, Milessa Silva ^[1] ; Cintra, Dennys Esper ^[2] ; Koike, Marcia ^[3] ; Nunes, Valeria Sutti ^[1] ; Demasi, Marina ^[1] ; Lin, Chin Jia ^[4] ; Masuzawa Beda, Lis Mie ^[1] ; Gioielli, Luiz Antonio ^[5] ; Assis Bombo, Renata de Paula ^[1] ; Machado, Roberta Marcondes ^[1] ; Catanozi, Sergio ^[1] ; Nakandakare, Edna Regina ^[1] ; Lottenberg, Ana Maria ^{[1, 6]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Lipides LIM10, BR-01246903 Sao Paulo, SP - Brazil

^[2] Univ Campinas UNICAMP, Sch Appl Sci, Lab Nutr Genom, BR-13484350 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med Sci, Emergency Care Res Unit Lab LIM51, BR-01246903 Sao Paulo - Brazil

^[4] Univ Sao Paulo, Fac Med Sci, Dept Pathol, Lab Mol Biol LIM22, BR-01246903 Sao Paulo - Brazil

^[5] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo - Brazil

^[6] Fac Israelita Ciencias Saude Albert Einstein, BR-05521200 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: NUTRIENTS; v. 11, n. 2 FEB 2019.
• Citações Web of Science: 2

Resumo

Interesterified fats are being widely used by the food industry in an attempt to replace trans fatty acids. The effect of interesterified fats containing palmitic or stearic acids on lipid metabolism and inflammatory signaling pathways in adipose and hepatic tissues was evaluated. Male LDLr-KO mice were fed a high-fat diet containing polyunsaturated (PUFA), palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR), or stearic interesterified (STEAR INTER) fats for 16 weeks. The expression of genes and protein levels involved in lipid metabolism and inflammatory processes in liver and white adipose tissue was determined by quantitative RT-PCR and by Western blot, respectively. The infiltration of inflammatory cells in hepatic and adipose tissues was determined by eosin and hematoxylin, while liver collagen content was determined by Sirius Red staining. Both interesterified fats increased liver collagen content and JNK phosphorylation. Additionally, the STEAR INTER group developed nonalcoholic steatohepatitis (NASH) associated with higher neutrophil infiltration. PALM INTER induced adipose tissue expansion and enlargement of adipocytes. Furthermore, PALM INTER triggered increased IKK phosphorylation and TNF protein content, conditions associated with the upstream activation of the NFkB signaling pathway. STEAR INTER induced NASH, while PALM INTER triggered hepatic fibrosis and adipocyte hypertrophy with inflammatory response in LDLr-KO mice. (AU)

• Processo FAPESP: 11/24083-7 - EFEITO DA INGESTÃO DA GORDURA INTERESTERIFICADA SOBRE VIAS INFLAMATÓRIAS E METABÓLICAS NO FÍGADO E TECIDO ADIPOSO DE CAMUNDONGOS LDLr-KO.
• Beneficiário: Maria Silvia Ferrari Lavrador
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/50249-2 - Efeito das gorduras interesterificadas sobre o metabolismo hepatico e adiposo e o desenvolvimento da aterosclerose em camundongos ldlr-ko.
• Beneficiário: Ana Maria Pita Lottenberg
• Modalidade de apoio: Auxílio à Pesquisa - Regular