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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Interesterified Fats Induce Deleterious Effects on Adipose Tissue and Liver in LDLr-KO Mice

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Ferrari Lavrador, Maria Silvia [1] ; Afonso, Milessa Silva [1] ; Cintra, Dennys Esper [2] ; Koike, Marcia [3] ; Nunes, Valeria Sutti [1] ; Demasi, Marina [1] ; Lin, Chin Jia [4] ; Masuzawa Beda, Lis Mie [1] ; Gioielli, Luiz Antonio [5] ; Assis Bombo, Renata de Paula [1] ; Machado, Roberta Marcondes [1] ; Catanozi, Sergio [1] ; Nakandakare, Edna Regina [1] ; Lottenberg, Ana Maria [1, 6]
Total Authors: 14
Affiliation:
[1] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Lipides LIM10, BR-01246903 Sao Paulo, SP - Brazil
[2] Univ Campinas UNICAMP, Sch Appl Sci, Lab Nutr Genom, BR-13484350 Sao Paulo - Brazil
[3] Univ Sao Paulo, Fac Med Sci, Emergency Care Res Unit Lab LIM51, BR-01246903 Sao Paulo - Brazil
[4] Univ Sao Paulo, Fac Med Sci, Dept Pathol, Lab Mol Biol LIM22, BR-01246903 Sao Paulo - Brazil
[5] Univ Sao Paulo, Fac Pharmaceut Sci, Dept Biochem & Pharmaceut Technol, BR-05508000 Sao Paulo - Brazil
[6] Fac Israelita Ciencias Saude Albert Einstein, BR-05521200 Sao Paulo, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: NUTRIENTS; v. 11, n. 2 FEB 2019.
Web of Science Citations: 2
Abstract

Interesterified fats are being widely used by the food industry in an attempt to replace trans fatty acids. The effect of interesterified fats containing palmitic or stearic acids on lipid metabolism and inflammatory signaling pathways in adipose and hepatic tissues was evaluated. Male LDLr-KO mice were fed a high-fat diet containing polyunsaturated (PUFA), palmitic (PALM), palmitic interesterified (PALM INTER), stearic (STEAR), or stearic interesterified (STEAR INTER) fats for 16 weeks. The expression of genes and protein levels involved in lipid metabolism and inflammatory processes in liver and white adipose tissue was determined by quantitative RT-PCR and by Western blot, respectively. The infiltration of inflammatory cells in hepatic and adipose tissues was determined by eosin and hematoxylin, while liver collagen content was determined by Sirius Red staining. Both interesterified fats increased liver collagen content and JNK phosphorylation. Additionally, the STEAR INTER group developed nonalcoholic steatohepatitis (NASH) associated with higher neutrophil infiltration. PALM INTER induced adipose tissue expansion and enlargement of adipocytes. Furthermore, PALM INTER triggered increased IKK phosphorylation and TNF protein content, conditions associated with the upstream activation of the NFkB signaling pathway. STEAR INTER induced NASH, while PALM INTER triggered hepatic fibrosis and adipocyte hypertrophy with inflammatory response in LDLr-KO mice. (AU)

FAPESP's process: 11/24083-7 - Effect of interesterified fat consumption in the inflammatory and metabolic pathways in the liver and adipose tissue of LDLr-KO mice.
Grantee:Maria Silvia Ferrari Lavrador
Support type: Scholarships in Brazil - Doctorate