Effects of two protease inhibitors from Bauhinia bauhinoides with different specificity towards gut enzymes of Nasutitermes corniger and its survival

Autor(es)

Ferreira, R. S. ^[1] ; Brito, V, M. ; Napoleao, T. H. ^[2] ; Silva, M. C. C. ^[3] ; Paiva, P. M. G. ^[2] ; Oliva, V, M. L.

Afiliação do(s) autor(es)

^[1] V, Univ Fed Sao Paulo, Biochem Dept, BR-04044020 Sao Paulo, SP - Brazil

^[2] Univ Fed Pernambuco, Biochem Dept, BR-50670420 Recife, PE - Brazil

^[3] Brito, M., V, V, Univ Fed Sao Paulo, Biochem Dept, BR-04044020 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 222, p. 364-370, MAY 2019.
• Citações Web of Science: 1

Resumo

Two recombinant protease inhibitors from Bauhinia bauhinioides, rBbKI (kallikrein inhibitor) and rBbCI (cruzipain inhibitor) were evaluated for insecticidal activity against workers and soldiers of Nasutitermes corniger (order: lsoptera; family: Termitidae) through the inhibitors' effect on the insect's gut enzymes. The inhibitor rBbKI was more effective than rBbCI in inhibiting the termite's gut enzymes. The kallikrein inhibitor showed termiticidal activity in workers with an LC50 of 0.9 mg mL(-1) after 4 days. Conversely, rBbKI did not affect the survival of soldiers and rBbCI did not show termiticidal activity against N. corniger. The two inhibitors showed different specificity towards the termite's gut enzymes, representing interesting tools to characterize N. corniger enzymes. The different effects of rBbKI and rBbCI on the termite's enzymes and survival may be linked to slight structural differences between these inhibitors. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 17/07972-9 - Desenvolvimento de compostos para profilaxia e tratamento de doenças cardiovasculares
• Beneficiário: Maria Luiza Vilela Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/06630-7 - Fragmentos derivados de proteínas com seletividade para inibição de enzimas de mamíferos e micro-organismos e seu papel como agente anti-inflamatório, antimicrobiano, antitrombótico e antitumoral: mecanismo de ação
• Beneficiário: Maria Luiza Vilela Oliva
• Modalidade de apoio: Auxílio à Pesquisa - Temático