Resveratrol-mediated reversal of changes in purinergic signaling and immune response induced by Toxoplasma gondii infection of neural progenitor cells

Autor(es):Mostrar menos -

Bottari, Nathieli B. ^{[1, 2, 3]} ; Pillat, Micheli M. ^[4] ; Schetinger, Maria R. C. ^{[1, 2]} ; Reichert, Karine P. ^{[1, 2]} ; Machado, Vanessa ^{[1, 2]} ; Assmann, Charles E. ^{[1, 2]} ; Ulrich, Henning ^[4] ; Dutra, Anielen ^{[1, 2]} ; Morsch, Vera M. ^{[1, 2]} ; Vidal, Tais ^{[1, 2]} ; Da Cruz, Ivana B. M. ^[5] ; Melazzo, Cinthia ^{[1, 2]} ; Da Silva, Aleksandro Schafer ^{[1, 2, 3, 6]}

Afiliação do(s) autor(es)

^[1] Univ Fed Santa Maria, Dept Biochem & Mol Biol, Santa Maria, RS - Brazil

^[2] Univ Fed Santa Maria, Grad Program Toxicol Biochem, Santa Maria, RS - Brazil

^[3] Univ Santa Catarina State, Dept Anim Sci, 680 D, Rua Beloni Trombeta Zanin, BR-89815630 Chapeco, SC - Brazil

^[4] Univ Sao Paulo, Inst Chem, Dept Biochem, Sao Paulo, SP - Brazil

^[5] Univ Fed Santa Maria, Grad Program Pharmacol, Santa Maria, RS - Brazil

^[6] UDESC, Grad Program Anim Sci, Chapeco, SC - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PURINERGIC SIGNALLING; v. 15, n. 1, p. 77-84, MAR 2019.
• Citações Web of Science: 1

Resumo

The effects of Toxoplasma gondii during embryonic development have not been explored despite the predilection of this parasite for neurons and glial cells. Here, we investigated the activation of the purinergic system and proinflammatory responses during congenital infection by T. gondii. Moreover, neuroprotective and neuromodulatory properties of resveratrol (RSV), a polyphenolic natural compound, were studied in infected neuronal progenitor cells (NPCs). For this study, NPCs were isolated from the telencephalon of infected mouse embryos and subjected to neurosphere culture in the presence of EGF and FGF2. ATP hydrolysis and adenosine deamination by adenosine deaminase activity were altered in conditions of T. gondii infection. P2X7 and adenosine A(2A) receptor expression rates were augmented in infected NPCs together with an increase of proinflammatory (INF- and TNF-) and anti-inflammatory (IL-10) cytokine gene expression. Our results confirm that RSV counteracted T. gondii-promoted effects on enzymes hydrolyzing extracellular nucleotides and nucleosides and also upregulated P2X7 and A(2A) receptor expression and activity, modulating INF-, TNF-, and IL-10 cytokine production, which plays an integral role in the immune response against T. gondii. (AU)

• Processo FAPESP: 12/50880-4 - Células-tronco: dos papéis de receptores de cininas e purinas às aplicações terapêuticas
• Beneficiário: Alexander Henning Ulrich
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/19478-3 - Papel do receptor B2 de cininas na patogênese e progressão da doença de Alzheimer família experimental: da neurogênese e resposta imune à cognição
• Beneficiário: Micheli Mainardi Pillat
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado