Evaluation of exercise-induced modulation of glial activation and dopaminergic damage in a rat model of Parkinson's disease using [C-11]PBR28 and [F-18]FDOPA PET

Autor(es)

Real, Caroline C. ^{[1, 2, 3]} ; Doorduin, Janine ^[1] ; Feltes, Paula Kopschina ^[1] ; Garcia, David Vallez ^[1] ; Faria, Daniele de Paula ^[3] ; Britto, Luiz R. ^[2] ; de Vries, Erik F. J. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Groningen, Univ Med Ctr Groningen, Dept Nucl Med & Mol Imaging, Groningen - Netherlands

^[2] Univ Sao Paulo, Dept Physiol & Biophys, Lab Cellular Neurobiol, Sao Paulo, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Lab Nucl Med LIM 43, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM; v. 39, n. 6, p. 989-1004, JUN 2019.
• Citações Web of Science: 7

Resumo

Evidence suggests that exercise can modulate neuroinflammation and neuronal damage. We evaluated if such effects of exercise can be detected with positron emission tomography (PET) in a rat model of Parkinson's disease (PD). Rats were unilaterally injected in the striatum with 6-hydroxydopamine (PD rats) or saline (controls) and either remained sedentary (SED) or were forced to exercise three times per week for 40 min (EX). Motor and cognitive functions were evaluated by the open field, novel object recognition, and cylinder tests. At baseline, day 10 and 30, glial activation and dopamine synthesis were assessed by {[}C-11]PBR28 and {[}F-18]FDOPA PET, respectively. PET data were confirmed by immunohistochemical analysis of microglial (Iba-1) / astrocyte (GFAP) activation and tyrosine hydroxylase (TH). {[}C-11]PBR28 PET showed increased glial activation in striatum and hippocampus of PD rats at day 10, which had resolved at day 30. Exercise completely suppressed glial activation. Imaging results correlated well with post-mortem Iba-1 staining, but not with GFAP staining. {[}F-18]FDOPA PET, TH staining and behavioral tests indicate that 6-OHDA caused damage to dopaminergic neurons, which was partially prevented by exercise. These results show that exercise can modulate toxin-induced glial activation and neuronal damage, which can be monitored noninvasively by PET. (AU)

• Processo FAPESP: 14/23509-9 - Imageamento por micro-PET da síntese de dopamina e do processo neuroinflamatório como forma de avaliação da redução do dano dopaminérgico induzida pelo exercício físico em um modelo da Doença de Parkinson em ratos
• Beneficiário: Caroline Cristiano Real Gregório
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Pós-Doutorado