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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Evaluation of exercise-induced modulation of glial activation and dopaminergic damage in a rat model of Parkinson's disease using [C-11]PBR28 and [F-18]FDOPA PET

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Author(s):
Real, Caroline C. [1, 2, 3] ; Doorduin, Janine [1] ; Feltes, Paula Kopschina [1] ; Garcia, David Vallez [1] ; Faria, Daniele de Paula [3] ; Britto, Luiz R. [2] ; de Vries, Erik F. J. [1]
Total Authors: 7
Affiliation:
[1] Univ Groningen, Univ Med Ctr Groningen, Dept Nucl Med & Mol Imaging, Groningen - Netherlands
[2] Univ Sao Paulo, Dept Physiol & Biophys, Lab Cellular Neurobiol, Sao Paulo, SP - Brazil
[3] Univ Sao Paulo, Sch Med, Lab Nucl Med LIM 43, Sao Paulo - Brazil
Total Affiliations: 3
Document type: Journal article
Source: JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM; v. 39, n. 6, p. 989-1004, JUN 2019.
Web of Science Citations: 7
Abstract

Evidence suggests that exercise can modulate neuroinflammation and neuronal damage. We evaluated if such effects of exercise can be detected with positron emission tomography (PET) in a rat model of Parkinson's disease (PD). Rats were unilaterally injected in the striatum with 6-hydroxydopamine (PD rats) or saline (controls) and either remained sedentary (SED) or were forced to exercise three times per week for 40 min (EX). Motor and cognitive functions were evaluated by the open field, novel object recognition, and cylinder tests. At baseline, day 10 and 30, glial activation and dopamine synthesis were assessed by {[}C-11]PBR28 and {[}F-18]FDOPA PET, respectively. PET data were confirmed by immunohistochemical analysis of microglial (Iba-1) / astrocyte (GFAP) activation and tyrosine hydroxylase (TH). {[}C-11]PBR28 PET showed increased glial activation in striatum and hippocampus of PD rats at day 10, which had resolved at day 30. Exercise completely suppressed glial activation. Imaging results correlated well with post-mortem Iba-1 staining, but not with GFAP staining. {[}F-18]FDOPA PET, TH staining and behavioral tests indicate that 6-OHDA caused damage to dopaminergic neurons, which was partially prevented by exercise. These results show that exercise can modulate toxin-induced glial activation and neuronal damage, which can be monitored noninvasively by PET. (AU)

FAPESP's process: 14/23509-9 - PET imaging of dopamine synthesis and neuroinflammation to evaluate the exercise-induced reduction in dopaminergic damage in a rat model of PD
Grantee:Caroline Cristiano Real Gregório
Support Opportunities: Scholarships abroad - Research Internship - Post-doctor