The impact of HER2 overexpression on the miRNA and circRNA transcriptomes in two breast cell lines and their vesicles

Autor(es)

de Amorim, Maria Galli ^{[1, 2]} ; Branco, Gabriela ^{[1, 2]} ; Valieris, Renan ^[3] ; Tarcitano, Emilio ^{[1, 2]} ; da Silva, Israel Tojal ^[3] ; de Araujo, Luiza Ferreira ^[1] ; Nunes, Diana Noronha ^[1] ; Dias-Neto, Emmanuel ^{[1, 4]}

Afiliação do(s) autor(es)

^[1] AC Camargo Canc Ctr, Lab Med Genom, Sao Paulo, SP - Brazil

^[2] Fundacao Antonio Prudente, Curso Posgrad Oncol, Sao Paulo, SP - Brazil

^[3] AC Camargo Canc Ctr, Lab Computat Biol, Sao Paulo, SP - Brazil

^[4] Univ Sao Paulo, Lab Neurociencias Alzira Denise Hertzog Silva LIM, Inst Psiquiatria, Fac Med, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PHARMACOGENOMICS; v. 20, n. 7, p. 493-502, MAY 2019.
• Citações Web of Science: 0

Resumo

HER2 upregulation is related with poor outcome in many tumor types. Whereas anti-HER2 treatment is the standard approach as adjuvant therapy in HER2-overexpressing breast cancer, the frequent relapses reinforce the need for alternative treatments. Here we used next-generation sequencing (NGS)to evaluate miRNAs and circRNAs in the cell-lines HB4a and C5.2, where the latter is a HER2-overexpressing clone of the former, and also from two different populations of their secreted extracellular vesicles. Whereas circRNA-levels were stable, we found at least 16 miRNAs apparently modulated by HER2-expression. The miR223-3p, miR-421 and miR-21-5p were validated in an independent cohort of 431 breast cancer patients from The Cancer Genome Atlas (TCGA). The consistent modulation of these molecules and their possible involvement in the HER2-axis makes them promising new targets to overcome HER2-activation. (AU)

• Processo FAPESP: 14/26897-0 - Epidemiologia e genômica de adenocarcinomas gástricos no Brasil
• Beneficiário: Emmanuel Dias-Neto
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 17/20278-4 - Detecção de marcadores de resistência e sensibilidade à quimioterapia em carcinoma de pulmão de pequenas células
• Beneficiário: Luíza Ferreira de Araújo
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 11/04399-0 - Descoberta e validação de pequenos RNAs preditivos de agressividade em câncer de mama
• Beneficiário: Gabriela Pereira Branco
• Modalidade de apoio: Bolsas no Brasil - Mestrado