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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The impact of HER2 overexpression on the miRNA and circRNA transcriptomes in two breast cell lines and their vesicles

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de Amorim, Maria Galli [1, 2] ; Branco, Gabriela [1, 2] ; Valieris, Renan [3] ; Tarcitano, Emilio [1, 2] ; da Silva, Israel Tojal [3] ; de Araujo, Luiza Ferreira [1] ; Nunes, Diana Noronha [1] ; Dias-Neto, Emmanuel [1, 4]
Total Authors: 8
[1] AC Camargo Canc Ctr, Lab Med Genom, Sao Paulo, SP - Brazil
[2] Fundacao Antonio Prudente, Curso Posgrad Oncol, Sao Paulo, SP - Brazil
[3] AC Camargo Canc Ctr, Lab Computat Biol, Sao Paulo, SP - Brazil
[4] Univ Sao Paulo, Lab Neurociencias Alzira Denise Hertzog Silva LIM, Inst Psiquiatria, Fac Med, Sao Paulo, SP - Brazil
Total Affiliations: 4
Document type: Journal article
Source: PHARMACOGENOMICS; v. 20, n. 7, p. 493-502, MAY 2019.
Web of Science Citations: 0

HER2 upregulation is related with poor outcome in many tumor types. Whereas anti-HER2 treatment is the standard approach as adjuvant therapy in HER2-overexpressing breast cancer, the frequent relapses reinforce the need for alternative treatments. Here we used next-generation sequencing (NGS)to evaluate miRNAs and circRNAs in the cell-lines HB4a and C5.2, where the latter is a HER2-overexpressing clone of the former, and also from two different populations of their secreted extracellular vesicles. Whereas circRNA-levels were stable, we found at least 16 miRNAs apparently modulated by HER2-expression. The miR223-3p, miR-421 and miR-21-5p were validated in an independent cohort of 431 breast cancer patients from The Cancer Genome Atlas (TCGA). The consistent modulation of these molecules and their possible involvement in the HER2-axis makes them promising new targets to overcome HER2-activation. (AU)

FAPESP's process: 14/26897-0 - Epidemiology and genomics of gastric adenocarcinomas in Brazil
Grantee:Emmanuel Dias-Neto
Support type: Research Projects - Thematic Grants
FAPESP's process: 17/20278-4 - Detection of drug resistance markers in small cell lung carcinoma
Grantee:Luíza Ferreira de Araújo
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 11/04399-0 - Discovery and validation of small RNAs predictive of aggressiveness in breast cancer
Grantee:Gabriela Pereira Branco
Support type: Scholarships in Brazil - Master