Discovery and validation of small RNAs predictive of aggressiveness in breast cancer

• Grant number: 11/04399-0
• Support Opportunities: Scholarships in Brazil - Master
• Start date: August 01, 2011
• End date: February 28, 2013
• Field of knowledge: Biological Sciences - Genetics - Human and Medical Genetics
• Principal Investigator: Emmanuel Dias-Neto
• Grantee: Gabriela Pereira Branco
• Host Institution: Hospital A C Camargo. Fundação Antonio Prudente (FAP). São Paulo , SP, Brazil

Abstract

Breast cancer is the women's second cause of deaths in the world. The few molecular markers used in the clinical breast cancer diagnosis are still not enough to allow the most adequate clinical and surgical treatment. The genetic heterogeneity presented in the patients and in the tumors also limit the prediction of clinical outcome during the evolution of the disease. In this sense, the search tumor aggressiveness markers are still very relevant. In the present project, we propose the investigation of the putative roles of small RNAs, including microRNAs (miRNAs), small nuclear RNAs (snRNAs) and small nucleolar RNAs (snoRNAs), as putative breast cancer markers of aggressiveness. Small RNAs isolated from 50 women's tumors with invasive ductal carcinoma (Her2+, PR-, ER-) will be evaluated by large-scale sequencing using the SOLiD 4.0 plataform. Tumor samples from two groups of women regarding aggressiveness and paired by age, lymph node status and citokeratins expression will be evaluated. Small RNA libraries will be tagged to allow later samples identification. The sequences obtained will be mapped and quantitatively and qualitatively evaluated, allowing the small RNA expression analysis of the tumor samples. The small RNAs with high and consistent differential expression will be confirmed by Real-Time PCR (qPCR) and validated in a breast cancer cell-line panel with distinct migration, invasion and metastasis properties. In this way, our goal is to contribute with the identification of new small RNAs and new putative aggressiveness breast cancer markers. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GABRIELA PEREIRA BRANCO; RENAN VALIERIS; LUCAS VENEZIAN POVOA; LUIZA FERREIRA DE ARAÚJO; GUSTAVO RIBEIRO FERNANDES; JORGE ESTEFANO SANTANA DE SOUZA; MARIA GALLI DE AMORIM; ELISA NAPOLITANO E FERREIRA; ISRAEL TOJAL DA SILVA; DIANA NORONHA NUNES; et al. A comparison between SOLiD 5500XLand Ion Torrent PGM-derived miRNA expression profiles in two breast cell lines. GENETICS AND MOLECULAR BIOLOGY, v. 43, n. 2, 2020-04-27. (11/04399-0, 14/26897-0)

DE AMORIM, MARIA GALLI; BRANCO, GABRIELA; VALIERIS, RENAN; TARCITANO, EMILIO; DA SILVA, ISRAEL TOJAL; DE ARAUJO, LUIZA FERREIRA; NUNES, DIANA NORONHA; DIAS-NETO, EMMANUEL. The impact of HER2 overexpression on the miRNA and circRNA transcriptomes in two breast cell lines and their vesicles. PHARMACOGENOMICS, v. 20, n. 7, p. 493-502, MAY 2019. (14/26897-0, 17/20278-4, 11/04399-0)

BRANCO, GABRIELA PEREIRA; VALIERIS, RENAN; POVOA, LUCAS VENEZIAN; DE ARAUJO, LUIZA FERREIRA; FERNANDES, GUSTAVO RIBEIRO; SANTANA DE SOUZA, JORGE ESTEFANO; DE AMORIM, MARIA GALLI; NAPOLITANO E FERREIRA, ELISA; DA SILVA, ISRAEL TOJAL; NUNES, DIANA NORONHA; et al. A comparison between SOLiD 5500XL-and Ion Torrent PGM-derived miRNA expression profiles in two breast cell lines. GENETICS AND MOLECULAR BIOLOGY, v. 43, n. 2, 2020. (11/04399-0, 14/26897-0)