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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Identification of DNA mutations in gastric washes from gastric adenocarcinoma patients: Possible implications for liquid biopsies and patient follow-up

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Autor(es):
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Pizzi, Melissa Pool [1] ; Bartelli, Thais Fernanda [1] ; Pelosof, Adriane Graicer [2] ; Freitas, Helano Carioca [3, 1] ; Begnami, Maria Dirlei [4] ; Senda de Abrantes, Lais Lie [5] ; Sztokfisz, Claudia [2] ; Valieris, Renan [6] ; Knebel, Franciele Hinterholz [7] ; Vaz Coelho, Luiz Gonzaga [8] ; da Costa, Wilson Luiz [9] ; Coimbra, Felipe J. F. [9] ; da Silva, Israel Tojal [6] ; de Amorim, Maria Galli [1] ; Nunes, Diana Noronha [1] ; Dias-Neto, Emmanuel [1, 10]
Número total de Autores: 16
Afiliação do(s) autor(es):
[1] AC Camargo Canc Ctr, Lab Med Genom, Tagua St, 440 Liberdade, Sao Paulo, SP - Brazil
[2] AC Camargo Canc Ctr, Endoscopy Sect, Sao Paulo, SP - Brazil
[3] AC Camargo Canc Ctr, Dept Clin Oncol, Sao Paulo, SP - Brazil
[4] AC Camargo Canc Ctr, Dept Pathol, Sao Paulo, SP - Brazil
[5] AC Camargo Canc Ctr, Int Res Ctr, Sao Paulo, SP - Brazil
[6] AC Camargo Canc Ctr, Lab Computat Biol, Sao Paulo, SP - Brazil
[7] Hosp Sirio Libanes, Ctr Oncol Mol, Sao Paulo, SP - Brazil
[8] Univ Fed Minas Gerais, Hosp Clin, Fac Med, Inst Alfa Gastroenterol, Belo Horizonte, MG - Brazil
[9] AC Camargo Canc Ctr, Dept Abdominal Surg, Sao Paulo, SP - Brazil
[10] Univ Sao Paulo, Fac Med, Inst Psiquiatria, Lab Neurosci Alzira Denise Hertzog Silva LIM 27, Sao Paulo, SP - Brazil
Número total de Afiliações: 10
Tipo de documento: Artigo Científico
Fonte: International Journal of Cancer; v. 145, n. 4, p. 1090-1098, AUG 15 2019.
Citações Web of Science: 3
Resumo

Whereas cancer patients have benefited from liquid biopsies, the scenario for gastric adenocarcinoma (GAC) is still dismal. We used next-generation deep sequencing of TP53-a highly mutated and informative gene in GAC-to assess mutations in tumor biopsies, plasma (PL) and stomach fluids (gastric wash-GW). We evaluated their potential to reveal tumor-derived mutations, useful for monitoring mutational dynamics at diagnosis, progression and treatment. Exon-capture libraries were constructed from 46 patients including tumor biopsies, GW and PL pre and post-treatment (196 samples), with high vertical coverage >8,000x. At diagnosis, we detected TP53 mutations in 15/46 biopsies (32.6%), 7/46 GW- (15.2%) and 6/46 PL-samples (13%). Biopsies and GW were concordant in 38/46 cases (82.6%) for the presence/absence of mutations and, furthermore, four GW-exclusive mutations were identified, suggesting tumor heterogeneity. Considering the combined analysis of GW and PL, TP53 mutations found in biopsies were also identified in 9/15 (60%) of cases, the highest detection level reported for GAC. Our study indicates that GW could be useful to track DNA alterations, especially if anchored to a comprehensive gene-panel designed for this malignancy. (AU)

Processo FAPESP: 14/26897-0 - Epidemiologia e genômica de adenocarcinomas gástricos no Brasil
Beneficiário:Emmanuel Dias-Neto
Linha de fomento: Auxílio à Pesquisa - Temático