Hypertonic solution-induced preconditioning reduces inflammation and mortality rate

Autor(es)

Pimentel, Rosangela Nascimento ^[1] ; Petroni, Ricardo Costa ^[1] ; Barbeiro, Hermes Vieira ^[1] ; Barbeiro, Denise Frediani ^[1] ; Andrade, Mariana Macedo ^[1] ; Ariga, Suely Kumini ^[1] ; Soriano, Francisco Garcia ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo FMUSP, Fac Med, Lab Invest Med LIM 51, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Emergencias Clin, Dept Clin Med, Fac Med, Av Dr Arnaldo 455, Room 3189, BR-01246903 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Inflammation-London; v. 16, JUL 3 2019.
• Citações Web of Science: 0

Resumo

BackgroundDysregulated inflammatory response is common cause of organ damage in critical care patients. Preconditioning/tolerance is a strategy to prevent exacerbated inflammation. The aim of this study is to analyze hypertonic saline 7.5% as a potential inducer of preconditioning that protect from a lethal dose of LPS and modulates systemic inflammatory profile in mice.MethodsMale Balb/C mice received intravenous (i.v.) injections of Hypertonic solution (NaCl 7.5%) (0.8ml) for 3days, on day 8th was challenged with LPS 15mg/kg. Controls with Saline 0.9%, urea and sorbitol were performed. Microarray of mRNA expression was analyzed from HS versus saline from macrophages to identified the pathways activated by HS.ResultsHS preconditioning reduced mortality after LPS injection as well reduced the cytokines release in plasma of the animals challenged by LPS. In order to check how HS induces a preconditioning state we measured plasma cytokines after each HS infusion. Repeated HS injections induced a state of preconditioning that reprograms the inflammatory response, resulting in reduced inflammatory cytokine production. A microarray of mRNA demonstrated that Hypertonic solution increased the expression of several genes in special Mapkbp1 and Atf3.Conclusionhypertonic solution induces preconditioning/tolerance reducing mortality and inflammatory response after LPS challenge. (AU)

• Processo FAPESP: 15/04138-2 - Sepse e tolerância ao Lipopolissacarídeo - papel da tolerância regulando o estresse oxidativo e suas consequências sobre a função mitocondrial, nitrosilação protéica e alterações do DNA
• Beneficiário: Francisco Garcia Soriano
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 09/03338-7 - Participação da célula B-1 na resposta inflamatória ao lipopolissacarídeo
• Beneficiário: Francisco Garcia Soriano
• Modalidade de apoio: Auxílio à Pesquisa - Regular