Raman spectroscopy of dorsal root ganglia from streptozotocin-induced diabetic neuropathic rats submitted to photobiomodulation therapy

Autor(es)

Vieira, Willians F. ^[1] ; de Magalhaes, Silviane F. ^[1] ; Farias, Felipe H. ^[1] ; de Thomaz, Andre A. ^[2] ; Parada, Carlos A. ^[1]

Afiliação do(s) autor(es)

^[1] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Lab Pain Studies, Campinas, SP - Brazil

^[2] State Univ Campinas UNICAMP, Inst Phys Gleb Wataghin, Dept Quantum Elect, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Biophotonics; v. 12, n. 11 JULY 2019.
• Citações Web of Science: 0

Resumo

In this study, we used Raman spectroscopy as a new tool to investigate pathological conditions at the level of chemical bond alterations in biological tissues. Currently, there have been no reports on the spectroscopic alterations caused by diabetic neuropathy in the dorsal root ganglia (DRG). DRG are a target for the treatment of neuropathic pain, and the need for more effective therapies is increasing. Photobiomodulation therapy (PBMT) through infrared low-level laser irradiation (904 nm) has shown analgesic effects on the treatment of neuropathy. Thus, the aim of this study was to use Raman spectroscopy to characterize the spectral DRG identities of streptozotocin (STZ)-induced diabetic neuropathic (hyperalgesic) rats and to study the influence of PBMT over such spectra. Characteristic DRG peaks were identified at 2704, 2850, 2885, 2940, 3061 and 3160 cm(-1), whose assignments are CH2/CH3 symmetric/asymmetric stretches, and CH vibrations of lipids and proteins. DRG from hyperalgesic rats showed an increased normalized intensity of 2704, 2850, 2885 and 3160 cm(-1). These same peaks had their normalized intensity reduced after PBMT treatment, accompanied by an anti-hyperalgesic effect. Raman spectroscopy was able to diagnose spectral alterations in DRG of hyperalgesic rats and the PBMT reduced the intensity of hyperalgesia and the altered Raman spectra. (AU)

• Processo FAPESP: 15/12673-5 - Efeitos da terapia laser de baixa intensidade (LLLT) em modelo de neuropatia diabética experimental
• Beneficiário: Willians Fernando Vieira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/25153-7 - Análise de expressão gênica diferencial de células do gânglio da raiz dorsal em modelo de diabetes e neuropatia diabética periférica
• Beneficiário: Carlos Amilcar Parada
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 18/05108-8 - Influência do laser de baixa intensidade nos neurônios: um estudo eletrofisiológico para ajudar a compreender os efeitos analgésicos do infravermelho na neuropatia diabética
• Beneficiário: Willians Fernando Vieira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado