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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Raman spectroscopy of dorsal root ganglia from streptozotocin-induced diabetic neuropathic rats submitted to photobiomodulation therapy

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Author(s):
Vieira, Willians F. [1] ; de Magalhaes, Silviane F. [1] ; Farias, Felipe H. [1] ; de Thomaz, Andre A. [2] ; Parada, Carlos A. [1]
Total Authors: 5
Affiliation:
[1] State Univ Campinas UNICAMP, Inst Biol, Dept Struct & Funct Biol, Lab Pain Studies, Campinas, SP - Brazil
[2] State Univ Campinas UNICAMP, Inst Phys Gleb Wataghin, Dept Quantum Elect, Campinas, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Journal of Biophotonics; v. 12, n. 11 JULY 2019.
Web of Science Citations: 0
Abstract

In this study, we used Raman spectroscopy as a new tool to investigate pathological conditions at the level of chemical bond alterations in biological tissues. Currently, there have been no reports on the spectroscopic alterations caused by diabetic neuropathy in the dorsal root ganglia (DRG). DRG are a target for the treatment of neuropathic pain, and the need for more effective therapies is increasing. Photobiomodulation therapy (PBMT) through infrared low-level laser irradiation (904 nm) has shown analgesic effects on the treatment of neuropathy. Thus, the aim of this study was to use Raman spectroscopy to characterize the spectral DRG identities of streptozotocin (STZ)-induced diabetic neuropathic (hyperalgesic) rats and to study the influence of PBMT over such spectra. Characteristic DRG peaks were identified at 2704, 2850, 2885, 2940, 3061 and 3160 cm(-1), whose assignments are CH2/CH3 symmetric/asymmetric stretches, and CH vibrations of lipids and proteins. DRG from hyperalgesic rats showed an increased normalized intensity of 2704, 2850, 2885 and 3160 cm(-1). These same peaks had their normalized intensity reduced after PBMT treatment, accompanied by an anti-hyperalgesic effect. Raman spectroscopy was able to diagnose spectral alterations in DRG of hyperalgesic rats and the PBMT reduced the intensity of hyperalgesia and the altered Raman spectra. (AU)

FAPESP's process: 15/12673-5 - Effects of low-level laser therapy (LLLT) in experimental diabetic neuropathy
Grantee:Willians Fernando Vieira
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 14/25153-7 - Analysis of differential gene expression of dorsal ganglion root cell in a model of diabetes and peripheral diabetic neuropathy
Grantee:Carlos Amilcar Parada
Support Opportunities: Regular Research Grants
FAPESP's process: 18/05108-8 - Direct influence of low-level laser irradiation on neurons: an electrophysiological study to helping comprise analgesic effects of infrared light in diabetic neuropathy
Grantee:Willians Fernando Vieira
Support Opportunities: Scholarships abroad - Research Internship - Doctorate