Genetic and chemical diversity among yacon [Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson] accessions based on iPBS markers and metabolomic fingerprinting

Autor(es)

Ziarovska, Jana ^[1] ; Padilla-Gonzalez, Guillermo F. ^[2] ; Viehmannova, Iva ^[3] ; Fernandez, Eloy ^[3]

Afiliação do(s) autor(es)

^[1] Slovak Univ Agr, Fac Agrobiol & Food Resources, Tr A Hlinku 2, Nitra 94976 - Slovakia

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Av Do Cafe, BR-14040903 Ribeirao Preto - Brazil

^[3] Czech Univ Life Sci Prague, Fac Trop AgriSci, Kamycka 129, Prague 16500 - Czech Republic

• Tipo de documento: Artigo Científico
• Fonte: Plant Physiology and Biochemistry; v. 141, p. 183-192, AUG 2019.
• Citações Web of Science: 1

Resumo

The present study is focused on the characterization of yacon {[}Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson] accessions from different geographic origins (Bolivia, Ecuador, and Peru) by iPBS markers and metabolomic fingerprinting. The results showed that the number of amplified polymorphic fragment levels ranged from 20 up to 27 with a level of polymorphism ranging from 80 to 100%. Five of the iPBS primers used in this study provided no specific banding pattern able to discriminate between the different yacon accessions. However, two iPBS primer pairs were able to separate Peru accessions from those of Ecuador and Bolivia. The UPLC-HRMS/MS-based metabolomic fingerprinting showed highly similar metabolomic fingerprints characterized by the accumulation of high quantities of sesquiterpene lactones and diterpenes, but no apparent geographic clustering. The present study demonstrates that yacon accessions from different geographical origins maintained ex situ (in the Czech Republic) present a rather low chemical and genetic diversity. (AU)

• Processo FAPESP: 14/17702-0 - Metabolômica e evolução de caracteres químicos na subtribo Espeletiinae (Asteraceae)
• Beneficiário: Guillermo Federico Padilla Gonzalez
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/26866-7 - Metabolômica, alvos enzimáticos e ferramentas in silico na busca por substâncias bioativas de plantas
• Beneficiário: Fernando Batista da Costa
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/21183-4 - Fingerprint metabólico e biossíntese de lactonas sesquiterpênicas em Smallanthus sonchifolius e Espeletia (Asteraceae): uma abordagem metabolômica e transcriptômica
• Beneficiário: Guillermo Federico Padilla Gonzalez
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado