Genetic and chemical diversity among yacon [Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson] accessions based on iPBS markers and metabolomic fingerprinting

Author(s)

Ziarovska, Jana ^[1] ; Padilla-Gonzalez, Guillermo F. ^[2] ; Viehmannova, Iva ^[3] ; Fernandez, Eloy ^[3]

Affiliation

^[1] Slovak Univ Agr, Fac Agrobiol & Food Resources, Tr A Hlinku 2, Nitra 94976 - Slovakia

^[2] Univ Sao Paulo, Sch Pharmaceut Sci Ribeirao Preto, Av Do Cafe, BR-14040903 Ribeirao Preto - Brazil

^[3] Czech Univ Life Sci Prague, Fac Trop AgriSci, Kamycka 129, Prague 16500 - Czech Republic

• Document type: Journal article
• Source: Plant Physiology and Biochemistry; v. 141, p. 183-192, AUG 2019.
• Web of Science Citations: 1

Abstract

The present study is focused on the characterization of yacon {[}Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson] accessions from different geographic origins (Bolivia, Ecuador, and Peru) by iPBS markers and metabolomic fingerprinting. The results showed that the number of amplified polymorphic fragment levels ranged from 20 up to 27 with a level of polymorphism ranging from 80 to 100%. Five of the iPBS primers used in this study provided no specific banding pattern able to discriminate between the different yacon accessions. However, two iPBS primer pairs were able to separate Peru accessions from those of Ecuador and Bolivia. The UPLC-HRMS/MS-based metabolomic fingerprinting showed highly similar metabolomic fingerprints characterized by the accumulation of high quantities of sesquiterpene lactones and diterpenes, but no apparent geographic clustering. The present study demonstrates that yacon accessions from different geographical origins maintained ex situ (in the Czech Republic) present a rather low chemical and genetic diversity. (AU)

• FAPESP's process: 14/17702-0 - Metabolomics and evolution of chemical characters in the subtribe Espeletiinae (Asteraceae)
• Grantee: Guillermo Federico Padilla Gonzalez
• Support Opportunities: Scholarships in Brazil - Doctorate
• FAPESP's process: 14/26866-7 - Metabolomics, enzymatic targets and in silico tools in the search of bioactive compounds from plants
• Grantee: Fernando Batista da Costa
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/21183-4 - Metabolic fingerprinting and biosynthesis of sesquiterpene lactones in Smallanthus sonchifolius and Espeletia (Asteraceae): a metabolomic and transcriptomic approach
• Grantee: Guillermo Federico Padilla Gonzalez
• Support Opportunities: Scholarships abroad - Research Internship - Doctorate