Metabolic fingerprinting and biosynthesis of sesquiterpene lactones in Smallanthus sonchifolius and Espeletia (Asteraceae): a metabolomic and transcriptomic approach

• Grant number: 16/21183-4
• Support type: Scholarships abroad - Research Internship - Doctorate
• Effective date (Start): April 01, 2017
• Effective date (End): March 31, 2018
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal researcher: Fernando Batista da Costa
• Grantee: Guillermo Federico Padilla Gonzalez
• Supervisor abroad: Otmar Spring
• Home Institution: Faculdade de Ciências Farmacêuticas de Ribeirão Preto (FCFRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
• Research place: University of Hohenheim, Germany
• Associated to the scholarship: 14/17702-0 - Metabolomics and evolution of chemical characters in the subtribe Espeletiinae (Asteraceae), BP.DR

Abstract

Smallanthus sonchifolius ("yacón") along with the genus Espeletia (Asteraceae, Millerieae) constitute closely related plant groups native to the South American Andes. These species display not only a remarkable genetic similarity, but also a uniform secondary metabolite chemistry, producing the same structural subtype of sesquiterpene lactones (STLs). This class of secondary metabolites possesses a broad range of biological activities and recent data suggest they are biosynthesized due to the adaptive advantages these compounds provide to the producing plants mainly as protective molecules. However, in spite of the potential adaptive roles STLs played in yacon and probably in members from the related genus Espeletia, their biosynthetic origin in recently diversified plant groups remain unexplored. Moreover, the lack of a comprehensive phytochemical characterization of S. sonchifolius across its ontogeny deserves further attention to understand the accumulation and possible adaptive roles of secondary metabolites in this species and others from the same tribe (Millerieae). Therefore, the aim of this research project is to study the metabolic fingerprint and biosynthesis of STLs across the ontogeny of S. sonchifolius and evaluate whether the ability to produce STLs is conserved in Espeletia, or if it is only restricted to a few species. Individuals will be harvested in different developmental stages and the LC-MS-based metabolome and expression of STL biosynthesis related genes will be assessed. Multivariate statistical analyses will be used to find putative changes in the metabolome of S. sonchifolius according to its ontogeny as well as to correlate metabolic data with gene expression studies in an integrated approach involving metabolomic and transcriptomic information. Sequencing of STLs genes in S. sonchifolius and Espeletia will allow finding regions of local similarity with sequences from different species of Asteraceae and phylogenetic reconstructions will employed to explore relationships among Asteraceae species based on genes from the secondary metabolism. (AU)

Scientific publications

(4)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

PADILLA-GONZALEZ, GUILLERMO E.; DIAZGRANADOS, MAURICIO; DA COSTA, FERNANDO B. Effect of the Andean Geography and Climate on the Specialized Metabolism of Its Vegetation: The Subtribe Espeletiinae (Asteraceae) as a Case Example. METABOLITES, v. 11, n. 4 APR 2021. Web of Science Citations: 0.

PADILLA-GONZALEZ, GUILLERMO F.; AMREHN, EVELYN; FREY, MAXIMILIAN; GOMEZ-ZELEDON, JAVIER; KAA, ALEVTINA; DA COSTA, FERNANDO B.; SPRING, OTMAR. Metabolomic and Gene Expression Studies Reveal the Diversity, Distribution and Spatial Regulation of the Specialized Metabolism of Yacon (Smallanthus sonchifolius, Asteraceae). INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 21, n. 12 JUN 2020. Web of Science Citations: 0.

PADILLA-GONZALEZ, GUILLERMO F.; FREY, MAXIMILIAN; GOMEZ-ZELEDON, JAVIER; DA COSTA, FERNANDO B.; SPRING, OTMAR. Metabolomic and gene expression approaches reveal the developmental and environmental regulation of the secondary metabolism of yacon (Smallanthus sonchifolius, Asteraceae). SCIENTIFIC REPORTS, v. 9, SEP 11 2019. Web of Science Citations: 0.

ZIAROVSKA, JANA; PADILLA-GONZALEZ, GUILLERMO F.; VIEHMANNOVA, IVA; FERNANDEZ, ELOY. Genetic and chemical diversity among yacon [Smallanthus sonchifolius (Poepp. et Endl.) H. Robinson] accessions based on iPBS markers and metabolomic fingerprinting. Plant Physiology and Biochemistry, v. 141, p. 183-192, AUG 2019. Web of Science Citations: 1.