Genome-wide association scan for QTL and their positional candidate genes associated with internal organ traits in chickens

Autor(es)

Monteiro Moreira, Gabriel Costa ^[1] ; Salvian, Mayara ^[1] ; Boschiero, Clarissa ^[1] ; Mello Cesar, Aline Silva ^[1] ; Reecy, James M. ^[2] ; Godoy, Thais Fernanda ^[1] ; Ledur, Monica Correa ^[3] ; Garrick, Dorian ^[4] ; Mourao, Gerson Barreto ^[1] ; Coutinho, Luiz L. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Luiz de Queiroz Coll Agr ESALQ, Sao Paulo - Brazil

^[2] Iowa State Univ, Dept Anim Sci, Ames, IA - USA

^[3] Embrapa Suinos & Aves, Concordia, SC - Brazil

^[4] Massey Univ, Sch Agr, Hamilton - New Zealand

• Tipo de documento: Artigo Científico
• Fonte: BMC Genomics; v. 20, n. 1 AUG 22 2019.
• Citações Web of Science: 0

Resumo

BackgroundPoultry breeding programs have been focused on improvement of growth and carcass traits, however, this has resulted in correlated changes in internal organ weights and increased incidence of metabolic disorders. These disorders can affect feed efficiency or even cause death. We used a high density SNP array (600K, Affymetrix) to estimate genomic heritability, perform genome-wide association analysis, and identify genomic regions and positional candidate genes (PCGs) associated with internal organ traits in an F2 chicken population. We integrated knowledge of haplotype blocks, selection signature regions and sequencing data to refine the list of PCGs.ResultsEstimated genomic heritability for internal organ traits in chickens ranged from low (LUNGWT, 0.06) to high (GIZZWT, 0.45). A total of 20 unique 1Mb windows identified on GGA1, 2, 4, 7, 12, 15, 18, 19, 21, 27 and 28 were significantly associated with intestine length, and weights or percentages of liver, gizzard or lungs. Within these windows, 14 PCGs were identified based on their biological functions: TNFSF11, GTF2F2, SPERT, KCTD4, HTR2A, RB1, PCDH7, LCORL, LDB2, NR4A2, GPD2, PTPN11, ITGB4 and SLC6A4. From those genes, two were located within haplotype blocks and three overlapped with selection signature regions. A total of 13,748 annotated sequence SNPs were in the 14 PCGs, including 156 SNPs in coding regions (124 synonymous, 26 non-synonymous, and 6 splice variants). Seven deleterious SNPs were identified in TNFSF11, NR4A2 or ITGB4 genes.ConclusionsThe results from this study provide novel insights to understand the genetic architecture of internal organ traits in chickens. The QTL detection performed using a high density SNP array covered the whole genome allowing the discovery of novel QTL associated with organ traits. We identified PCGs within the QTL involved in biological processes that may regulate internal organ growth and development. Potential functional genetic variations were identified generating crucial information that, after validation, might be used in poultry breeding programs to reduce the occurrence of metabolic disorders. (AU)

• Processo FAPESP: 14/21380-9 - Estudo de associação ampla do genoma para deposição de gordura e rendimento de carcaça em galinhas
• Beneficiário: Gabriel Costa Monteiro Moreira
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/00569-1 - Imputação e associação genômica ampla utilizando haplótipos para gordura abdominal em galinhas.
• Beneficiário: Gabriel Costa Monteiro Moreira
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/08704-0 - Identificação de locos de interesse zootécnico na galinha doméstica
• Beneficiário: Luiz Lehmann Coutinho
• Modalidade de apoio: Auxílio à Pesquisa - Temático