Inhibition of MAPK and STAT3-SOCS3 by Sakuranetin Attenuated Chronic Allergic Airway Inflammation in Mice

Autor(es):Mostrar menos -

Santana, Fernanda P. R. ^{[1, 2]} ; da Silva, Rafael C. ^{[1, 3]} ; Grecco, Simone dos S. ^[4] ; Pinheiro, Aruana J. M. C. R. ^{[5, 6]} ; Caperuto, Luciana C. ^[1] ; Arantes-Costa, Fernanda M. ^[2] ; Claudio, Samuel R. ^[3] ; Yoshizaki, Kelly ^[7] ; Macchione, Mariangela ^[7] ; Ribeiro, Daniel A. ^[3] ; Tiberio, Iolanda F. L. C. ; Lima-Neto, Lidio G. ^{[5, 6]} ; Lago, Joao H. G. ^[8] ; Prado, Carla M. ^{[1, 3, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Dept Biol Sci, Diadema, SP - Brazil

^[2] Univ Sao Paulo, Sch Med, Dept Med, Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Biosci, Santos - Brazil

^[4] Univ Anhanguera Sao Paulo, Sao Paulo, SP - Brazil

^[5] Univ CEUMA, Sao Luis, MA - Brazil

^[6] Programa Posgrad Rede BIONORTE, Rio Branco - Brazil

^[7] Univ Sao Paulo, Sch Med, Dept Pathol, Sao Paulo, SP - Brazil

^[8] Fed Univ ABC, Ctr Nat Sci & Humanities, Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Mediators of Inflammation; v. 2019, SEP 3 2019.
• Citações Web of Science: 0

Resumo

Asthma allergic disease is caused by airway chronic inflammation. Some intracellular signaling pathways, such as MAPK and STAT3-SOCS3, are involved in the control of airway inflammation in asthma. The flavonoid sakuranetin demonstrated an anti-inflammatory effect in different asthma models. Our aim was to clarify how sakuranetin treatment affects MAPK and STAT3-SOCS3 pathways in a murine experimental asthma model. Mice were submitted to an asthma ovalbumin-induction protocol and were treated with vehicle, sakuranetin, or dexamethasone. We assayed the inflammatory profile, mucus production, and serum antibody, STAT3-SOCS3, and MAPK levels in the lungs. Morphological alterations were also evaluated in the liver. LPS-stimulated RAW 264.7 cells were used to evaluate the effects of sakuranetin on nitric oxide (NO) and cytokine production. In vivo, sakuranetin treatment reduced serum IgE levels, lung inflammation (eosinophils, neutrophils, and Th2/Th17 cytokines), and respiratory epithelial mucus production in ovalbumin-sensitized animals. Considering possible mechanisms, sakuranetin inhibits the activation of ERK1/2, JNK, p38, and STAT3 in the lungs. No alterations were found in the liver for treated animals. Sakuranetin did not modify in vitro cell viability in RAW 264.7 and reduced NO release and gene expression of IL-1 beta and IL-6 induced by LPS in these cells. In conclusion, our data showed that the inhibitory effects of sakuranetin on eosinophilic lung inflammation can be due to the inhibition of Th2 and Th17 cytokines and the inhibition of MAPK and STAT3 pathways, reinforcing the idea that sakuranetin can be considered a relevant candidate for the treatment of inflammatory allergic airway disease. (AU)

• Processo FAPESP: 18/06088-0 - Efeito do tratamento com eugenol e deidrodieugenol isolados de Nectandra leucantha (Lauraceae) em modelos experimentais de doenças pulmonares agudas e crônicas.
• Beneficiário: Carla Máximo Prado
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/25689-4 - Efeito do sistema colinérgico na inflamação pulmonar aguda e crônica.
• Beneficiário: Carla Máximo Prado
• Modalidade de apoio: Auxílio à Pesquisa - Regular