Presence of circulating folic acid in plasma and its relation with dietary intake, vitamin B complex concentrations and genetic variants

Autor(es)

Steluti, Josiane ^[1] ; Reginaldo, Christina ^[2] ; Selhub, Jacob ^[2] ; Paul, Ligi ^[2] ; Fisberg, Regina Mara ^[1] ; Marchioni, Dirce Maria ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Sch Publ Hlth, Dept Nutr, BR-01246904 Sao Paulo - Brazil

^[2] Tufts Univ, Jean Mayer USDA Human Nutr Res Ctr Aging, Boston, MA 02111 - USA

• Tipo de documento: Artigo Científico
• Fonte: EUROPEAN JOURNAL OF NUTRITION; v. 58, n. 8, p. 3069-3077, DEC 2019.
• Citações Web of Science: 2

Resumo

PurposeThe aim of this study was to investigate circulating folic acid (FA) and predict circulating FA concentrations in the population related to dietary intake, vitamin concentrations, and interaction with the genetic variants involved in folate metabolism.MethodsData were from the `Health Survey of SAo Paulo' a cross-sectional population-based survey, conducted in SAo Paulo City, Brazil. The participants (n=750) provided fasting blood samples and food intake data. Folate, homocysteine, and B6 and B12 vitamins were assayed. DNA was isolated, and the genotypes for polymorphisms involved in folate metabolism were determined. A generalized linear model was performed to predict circulating FA concentration.ResultsThe circulating FA was detected in 80.0% of the population, with a median concentration of 1.6 nmol/L (IQR 0.5-2.9). The increase of circulating FA concentrations was directly associated with total folate concentration (beta coeff. 1.03; 95% CI 1.02-1.04), age (beta coeff. 1.01; 95% CI 1.01-1.02), current smoker (beta coeff. 1.51; 95% CI 1.16-1.97), self-reported skin color (beta coeff. 1.83; 95% CI 1.51-2.20), as well as interaction between folate concentration and 19-bp deletion polymorphism in DHFR (beta coeff. 1.02; 95% CI 1.01-1.03), and inversely associated with vitamin B6 (beta coeff. 0.99; 95% CI 0.98-0.99).ConclusionsIn the current study, the presence of detectable circulating folic acid is high, and its concentration is elevated compared with other populations. Age, smoking, lower concentration of vitamin B6 and genetic variant are associated with increased levels of circulating FA. Further researches are needed to acknowledge and guarantee the safety of exposure to folic acid, especially in countries which have mandatory fortification. (AU)

• Processo FAPESP: 15/12196-2 - Presença de ácido fólico não metabolizado e associações com metilação global de dna, estresse oxidativo, marcadores inflamatórios e assinatura metabólica: análise após a fortificação mandatória de farinhas com ácido fólico
• Beneficiário: Josiane Steluti
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/05505-0 - Padronização de metodologia e análise de ácido fólico não metabolizado após a fortificação mandatória com ácido fólico: estudo de base populacional no Município de São Paulo
• Beneficiário: Josiane Steluti
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 11/19788-1 - Avaliação da deficiência de folato após a fortificação mandatória de ácido fólico e consequências à saúde: estudo de base populacional no município de São Paulo
• Beneficiário: Dirce Maria Lobo Marchioni
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/19899-5 - Consumo dietético, variantes genéticas e relação com os níveis sanguíneos de folato, ácido fólico não metabolizado e homocisteína após a fortificação mandatória de ácido fólico: estudo de base populacional - ISA-Capital.
• Beneficiário: Josiane Steluti
• Modalidade de apoio: Bolsas no Brasil - Doutorado