Rapid, sensitive and simultaneous determination of 16 endocrine-disrupting chemicals (parabens, benzophenones, bisphenols, and triclocarban) in human urine based on microextraction by packed sorbent combined with liquid chromatography tandem mass spectrometry (MEPS-LC-MS/MS)

Autor(es)

Silveira, Romena Sanglard ^[1] ; Rocha, Bruno Alves ^{[2, 1]} ; Rodrigues, Jairo Lisboa ^[3] ; Barbosa, Jr., Fernando ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Toxicol & Essencialidade Metais, Ave Cafe S-N, BR-14049903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP - Brazil

^[3] Univ Fed Vales Jequitinhonha & Mucuri, Lab Multiusuario, Inst Ciencia Engn & Tecnol, BR-39803371 Teofilo Otoni, MG - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Chemosphere; v. 240, FEB 2020.
• Citações Web of Science: 0

Resumo

A high demand exists in human biomonitoring studies for reliable and straightforward methods that generate data faster and simultaneously. Thus, the present study combines microextraction by packed sorbent (MEPS) and liquid chromatography coupled to mass spectrometry (LC-MS/MS) for simultaneous extraction and determination of various classes of endocrine-disrupting chemicals (EDCs), including parabens, benzophenones, bisphenols, and the antimicrobial, triclocarban in human urine samples. Optimized MEPS conditions were: i) MEPS sorbent (C18), ii) pH of sample (3), iii) volume of sample (250 mu L), iv) number of draws-eject cycles (5) and (vi) desorption solvent conditions (100 mu L of CH3OH:H2O 80:20 v/v). The calibration curves were linear over the selected ranges for all studied compound, with correlation coefficients higher than 0.99. The variation coefficient for precision was lower than 20% at lower concentrations and lower than 15% at the higher concentrations studied. The accuracy ranged from 90% to 118%. The proposed strategy affords several advantages over currently published approaches, including simplicity of operation and reduction of sample and solvent volumes and time for matrix clean-up. Moreover, the analytical performance of each MEPS cartridge remained stable over the analysis of at least 70 samples (RSD < 10%). Thus, the current procedure may be an interesting high-throughput alternative for large routine human biomonitoring studies. Urinary geometric mean concentrations of EDCs obtained in this study were close than those previously reported for Brazilian children. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 14/50867-3 - INCT 2014: Instituto Nacional de Ciência e Tecnologia de Bioanalítica
• Beneficiário: Marco Aurelio Zezzi Arruda
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 18/24069-3 - Do biomonitoramento ao reconhecimento de assinaturas do exposoma humano visando antecipar riscos para uma saúde contínua
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 14/19914-5 - Impacto da exposição transplacentária ao fumo do tabaco no DNA do recém-nascido: avaliação de dano genético e alterações epigenéticas
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/02949-6 - Desenvolvimento de métodos visando a especiação química de Hg e As em amostras ambientais e biológicas por HPLC-ICP-MS utilizando preparo de amostra por microextração em sorvente empacotado (MEPS)
• Beneficiário: Fernando Barbosa Júnior
• Modalidade de apoio: Auxílio à Pesquisa - Regular
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• Beneficiário: Bruno Alves Rocha
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado