Rapid, sensitive and simultaneous determination of 16 endocrine-disrupting chemicals (parabens, benzophenones, bisphenols, and triclocarban) in human urine based on microextraction by packed sorbent combined with liquid chromatography tandem mass spectrometry (MEPS-LC-MS/MS)

Author(s)

Silveira, Romena Sanglard ^[1] ; Rocha, Bruno Alves ^{[2, 1]} ; Rodrigues, Jairo Lisboa ^[3] ; Barbosa, Jr., Fernando ^[1]

Affiliation

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Lab Toxicol & Essencialidade Metais, Ave Cafe S-N, BR-14049903 Ribeirao Preto, SP - Brazil

^[2] Univ Fed Sao Paulo, Inst Ciencias Ambientais Quim & Farmaceut, Rua Prof Artur Riedel 275, BR-09972270 Diadema, SP - Brazil

^[3] Univ Fed Vales Jequitinhonha & Mucuri, Lab Multiusuario, Inst Ciencia Engn & Tecnol, BR-39803371 Teofilo Otoni, MG - Brazil

• Document type: Journal article
• Source: Chemosphere; v. 240, FEB 2020.
• Web of Science Citations: 0

Abstract

A high demand exists in human biomonitoring studies for reliable and straightforward methods that generate data faster and simultaneously. Thus, the present study combines microextraction by packed sorbent (MEPS) and liquid chromatography coupled to mass spectrometry (LC-MS/MS) for simultaneous extraction and determination of various classes of endocrine-disrupting chemicals (EDCs), including parabens, benzophenones, bisphenols, and the antimicrobial, triclocarban in human urine samples. Optimized MEPS conditions were: i) MEPS sorbent (C18), ii) pH of sample (3), iii) volume of sample (250 mu L), iv) number of draws-eject cycles (5) and (vi) desorption solvent conditions (100 mu L of CH3OH:H2O 80:20 v/v). The calibration curves were linear over the selected ranges for all studied compound, with correlation coefficients higher than 0.99. The variation coefficient for precision was lower than 20% at lower concentrations and lower than 15% at the higher concentrations studied. The accuracy ranged from 90% to 118%. The proposed strategy affords several advantages over currently published approaches, including simplicity of operation and reduction of sample and solvent volumes and time for matrix clean-up. Moreover, the analytical performance of each MEPS cartridge remained stable over the analysis of at least 70 samples (RSD < 10%). Thus, the current procedure may be an interesting high-throughput alternative for large routine human biomonitoring studies. Urinary geometric mean concentrations of EDCs obtained in this study were close than those previously reported for Brazilian children. (C) 2019 Elsevier Ltd. All rights reserved. (AU)

• FAPESP's process: 14/50867-3 - INCT 2014: National Institute of Science and Technology in Bioanalysis
• Grantee: Marco Aurelio Zezzi Arruda
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 18/24069-3 - ReSEARCH: Recognizing Signatures of the Exposome to Anticipate the Risks for a Continuous Health
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Research Projects - Thematic Grants
• FAPESP's process: 14/19914-5 - Impact of transplacental exposure to tobacco smoke in the DNA of newborn: genetic and epigenetic effects
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 16/02949-6 - The development of analytical procedures for Hg and as speciation in environmental and biological samples by HPLC-ICP-MS and sample preparation with microextraction by packed sorbent (MEPS)
• Grantee: Fernando Barbosa Júnior
• Support Opportunities: Regular Research Grants
• FAPESP's process: 13/23710-3 - The use of Shpol'skii spectroscopy and LC-MS/MS for determination of bisphenol A and analogues in urine samples and exposure assessment in children living in the São Paulo State, Brazil
• Grantee: Bruno Alves Rocha
• Support Opportunities: Scholarships in Brazil - Post-Doctoral