Availability of arsenic in rice grains by in vitro and in vivo (humans) assays

Autor(es)

Pedron, Tatiana ^[1] ; Freire, Bruna Moreira ^[1] ; Castro, Carlos Eduardo ^[1] ; Ribal, Luiz Felipe ^[2] ; Batista, Bruno Lemos ^[1]

Afiliação do(s) autor(es)

^[1] Fed Univ ABC, Ctr Nat & Human Sci, BR-09210580 Santo Andre, SP - Brazil

^[2] Fed Univ ABC, Ctr Engn Modeling & Appl Social Sci, BR-09210580 Santo Andre, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY; v. 56, p. 184-191, DEC 2019.
• Citações Web of Science: 0

Resumo

Background: Rice grains are consumed by approximately half of the world's population. This cereal has higher arsenic (As) concentrations in grains than wheat or barley. Arsenic determination in food and/or in vitro studies are important for risk assessment; however, it is not enough to assess the real human exposure. Method: In vitro bioaccessibility was carried out in husked-rice using gastric and intestinal solutions similar to humans. Also, As naturally found in husked-rice was evaluated by in vivo bioavailability in humans. For this purpose, diets from the 1st and 2nd days were free of foods known to be high in As; 3rd and 4th days the diets were composed by rice and water and; 5th and 6th the diet was similar the 1st and 2nd days. During all experimentation, a representative aliquot of each meal, blood and urine were collected for total As (t-As) determination. Arsenic species were determined in the urine. Results: t-As in husked rice varied from 157.3 +/- 30.6 to 240.2 +/- 85.2 mu g kg(-1). The in vitrobioaccessible fractions ranged from 91 to 94%. Inorganic As (i-As) ranged from 99.7 +/- 11.2 to 159.5 +/- 29.4 mu g kg(-1). For the in vivo assay, t-As concentrations in the woman and man blood were about 3 mu g mL(-1) from the 1st to 6th day. Arsenic from the rice ingested was excreted by urine about 72 h after ingestion. The t-As and dimethyl As (DMA) in urine ranged from 3.59 to 47.17 and 1.02 to 2.55 mu g g(-1) creatinine for the volunteers, indicating a two-fold DMA-increase in urine after ingestion of husked-rice. Conclusion: After rice ingestion, As was quickly metabolized. The higher As concentrations were found in urine 72 h after rice ingestion. The main As-specie found in urine was DMA, indicating that methylation of As from rice followed by urine excretion is the main biological pathway for As excretion. (AU)

• Processo FAPESP: 17/20914-8 - Validação de método para determinação de elementos traço em urina para avaliação ocupacional de trabalhadores de fundição
• Beneficiário: Bruna Moreira Freire
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 16/10060-9 - Equipamento multi-usuário (EMU) concedido no Processo FAPESP 2014/05151-0: espectrômetro de massas com plasma indutivamente acoplado hifenado a um cromatógrafo a líquido de alta eficiência (HPLC-ICP-MS)
• Beneficiário: Bruno Lemos Batista
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 14/05151-0 - Arsênio e arroz: monitoramento e estudos de (bio)remediação para segurança alimentar
• Beneficiário: Bruno Lemos Batista
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 16/19924-6 - Avaliação da bioacessibilidade in vitro e da biodisponibilidade in vivo de arsênio ingerido através do arroz comumente consumido por brasileiros
• Beneficiário: Tatiana Pedron
• Modalidade de apoio: Bolsas no Brasil - Doutorado