Acute thiamethoxam toxicity in honeybees is not enhanced by common fungicide and herbicide and lacks stress-induced changes in mRNA splicing

Autor(es)

Decio, Pamela ^[1] ; Ustaoglu, Pinar ^{[2, 3, 4]} ; Roat, Thaisa C. ^[1] ; Malaspina, Osmar ^[1] ; Devaud, Jean-Marc ^[5] ; Stoger, Reinhard ^[6] ; Soller, Matthias ^[2]

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, UNESP, Inst Biociencias, Ctr Estudos Insetos Sociais, Rio Claro, SP - Brazil

^[2] Univ Birmingham, Coll Life & Environm Sci, Sch Biosci, Birmingham B15 2TT, W Midlands - England

^[3] Imperial Coll London, MRC Ctr Mol Bacteriol & Infect, Ground Floor, Flowers Bldg, South Kensington Campus, London SW7 2AZ - England

^[4] Imperial Coll London, Dept Life Sci, Ground Floor, Flowers Bldg, South Kensington Campus, London SW7 2AZ - England

^[5] Toulouse Univ, Ctr Integrat Biol, Res Ctr Anim Cognit, CNRS, UPS, Toulouse - France

^[6] Univ Nottingham, Sch Biosci, Nottingham Sutton Bonington Campus, Loughborough LE12 5RD, Leics - England

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 9, DEC 16 2019.
• Citações Web of Science: 0

Resumo

Securing food supply for a growing population is a major challenge and heavily relies on the use of agrochemicals to maximize crop yield. It is increasingly recognized, that some neonicotinoid insecticides have a negative impact on non-target organisms, including important pollinators such as the European honeybee Apis mellifera. Toxicity of neonicotinoids may be enhanced through simultaneous exposure with additional pesticides, which could help explain, in part, the global decline of honeybee colonies. Here we examined whether exposure effects of the neonicotinoid thiamethoxam on bee viability are enhanced by the commonly used fungicide carbendazim and the herbicide glyphosate. We also analysed alternative splicing changes upon pesticide exposure in the honeybee. In particular, we examined transcripts of three genes: (i) the stress sensor gene X box binding protein-1 (Xbp1), (ii) the Down Syndrome Cell Adhesion Molecule (Dscam) gene and iii) the embryonic lethal/abnormal visual system (elav) gene, which are important for neuronal function. Our results showed that acute thiamethoxam exposure is not enhanced by carbendazim, nor glyphosate. Toxicity of the compounds did not trigger stress-induced, alternative splicing in the analysed mRNAs, thereby leaving dormant a cellular response pathway to these man-made environmental perturbations. (AU)

• Processo FAPESP: 15/22368-5 - Análise da expressão dos genes ELAV e Dscam em Apis mellifera (Hymenoptera: Apidae) expostas ao tiametoxam
• Beneficiário: Pâmela Decio Horst
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/23197-7 - Estresse celular e atividade de enzimas biomarcadoras em abelhas africanizadas Apis mellifera Lineu, 1758 (Hymenoptera, Apidae) expostas ao tiametoxam
• Beneficiário: Pâmela Decio Horst
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/13370-8 - Como o cérebro de Apis mellifera (Lineu, 1758) (Hymenoptera, Apidae) responde à dose subletal de tiametoxam?
• Beneficiário: Thaisa Cristina Roat
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores