Comparing Data-Independent Acquisition and Parallel Reaction Monitoring in Their Abilities To Differentiate High-Density Lipoprotein Subclasses

Autor(es)

Silva, Amanda R. M. ^[1] ; Toyoshima, Marcos T. K. ^{[2, 3]} ; Passarelli, Marisa ^{[2, 4]} ; Di Mascio, Paolo ^[1] ; Ronsein, Graziella E. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05513970 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Fac Med, Hosp Clin, Lab Lipides LIM 10, BR-01246903 Sao Paulo - Brazil

^[3] Univ Sao Paulo, Fac Med, Hosp Clin, Serv Oncoendocrinol, Inst Canc Estado Sao Paulo Oc, BR-01246000 Sao Paulo - Brazil

^[4] Univ Nove Julho, Programa Posgrad, BR-01504001 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PROTEOME RESEARCH; v. 19, n. 1, p. 248-259, JAN 2020.
• Citações Web of Science: 0

Resumo

High-density lipoprotein (HDL) is a diverse group of particles with multiple cardioprotective functions. HDL proteome follows HDL particle complexity. Many proteins were described in HDL, but consistent quantification of HDL protein cargo is still a challenge. To address this issue, the aim of this work was to compare data-independent acquisition (DIA) and parallel reaction monitoring (PRM) methodologies in their abilities to differentiate HDL subclasses through their proteomes. To this end, we first evaluated the analytical performances of DIA and PRM using labeled peptides in pooled digested HDL as a biological matrix. Next, we compared the quantification capabilities of the two methodologies for 24 proteins found in HDL2 and HDL3 from 19 apparently healthy subjects. DIA and PRM exhibited comparable linearity, accuracy, and precision. Moreover, both methodologies worked equally well, differentiating HDL subclasses' proteomes with high precision. Our findings may help to understand HDL functional diversity. (AU)

• Processo FAPESP: 17/07725-1 - Proteômica: uma ferramenta para a investigação da composição e função da HDL em hiperlipidemia
• Beneficiário: Amanda Ribeiro Martins da Silva
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 16/00696-3 - Compreendendo composição e função da HDL através de proteômica
• Beneficiário: Graziella Eliza Ronsein
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 13/07937-8 - Redoxoma
• Beneficiário: Ohara Augusto
• Modalidade de apoio: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs